Multiplex Assay Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay)

HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 101
EDTA plasma(n=5) 80-88 83
heparin plasma(n=5) 78-102 97
sodium citrate plasma(n=5) 79-93 79

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 80-92% 83-92% 79-98%
EDTA plasma(n=5) 97-105% 94-102% 79-95% 89-97%
heparin plasma(n=5) 79-92% 83-91% 96-105% 89-97%
sodium citrate plasma(n=5) 80-101% 95-104% 91-99% 84-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:HBEGF) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Neuropathology Astrocyte ERK phosphorylation precedes K+-induced swelling but follows hypotonicity-induced swelling. PubMed: 21118399
Toxicological Sciences Cypermethrin Induces Astrocyte Apoptosis by the Disruption of the Autocrine/Paracrine Mode of Epidermal Growth Factor Receptor Signaling Pubmed: source
PAIN RESEARCH 軽度炎症性膀胱痛マウスモデルの構築とNGFの関与 Jstage:Source
European Journal of Pain A novel method for assessing bladder-related pain reveals the involvement of nerve growth factor in painassociated with cyclophosphamide-induced chronic cystitis in mice. Pubmed:25820250
Kinki daigaku 2 型糖尿病モデルラットにおける糖尿病性腎症の進行とHB-EGF の関連性 17844
laboratory investigation Heparin-binding epidermal growth factor contributes to COPD disease severity by modulating airway fibrosis and pulmonary epithelial–mesenchymal transition Pubmed:29581578
International Journal of Biological Sciences CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17
Redox Biology Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth Pubmed: 30769285
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets Pubmed: 33164313
象牙芽細胞の枯渇は象牙芽細胞分化と象牙質形成を誘導する
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APB479Hu01 Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Cell culture; Activity Assays.
APB479Hu61 Active Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Cell culture; Activity Assays.
EPB479Hu61 Eukaryotic Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Positive Control; Immunogen; SDS-PAGE; WB.
RPB479Hu01 Recombinant Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Positive Control; Immunogen; SDS-PAGE; WB.
PAB479Hu01 Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB,IHC
LAB479Hu71 Biotin-Linked Polyclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC.
MAB479Hu22 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC; IP.
MAB479Hu25 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC; IP.
MAB479Hu21 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC; IP.
MAB479Hu23 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC; IP.
MAB479Hu24 Monoclonal Antibody to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) WB; IHC; ICC; IP.
SEB479Hu ELISA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB479Hu CLIA Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) Chemiluminescent immunoassay for Antigen Detection.
LMB479Hu Multiplex Assay Kit for Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.