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Multiplex Assay Kit for Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay)
PL; Plasmin; Activation peptide; Angiostatin
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB236Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypePlasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 349
US$ 363
US$ 383
US$ 410
US$ 437
US$ 477
US$ 538
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 88-96 | 93 |
EDTA plasma(n=5) | 80-92 | 85 |
heparin plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 99-105% | 83-103% | 83-93% | 89-98% |
EDTA plasma(n=5) | 80-97% | 92-105% | 91-101% | 89-103% |
heparin plasma(n=5) | 84-101% | 97-104% | 99-105% | 87-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:Plg) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
International Journal of Molecular Sciences | The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC Mdpi: Source |
J Anim Physiol Anim Nutr (Berl). | Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season. pubmed:28084661 |
Neurotherapeutics | iTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management pubmed:29247448 |
Veterinarski Arhiv | The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid … 10.24099:vet.arhiv.170113 |
Food Bioscience | Reducing antigenicity of ¦Â-lactoglobulin, probiotic properties and safety evaluation of Lactobacillus plantarum AHQ-14 and Lactobacillus bulgaricus BD0390 |
Foods | Antigenicity and Safety Evaluation of Lactiplantibacillus plantarum 7-2 Screened to Reduce α-Casein Antigen Pubmed:35010214 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
NPB236Hu01 | Native Plasminogen (Plg) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB236Hu03 | Recombinant Plasminogen (Plg) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB236Hu01 | Recombinant Plasminogen (Plg) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB236Hu01 | Active Plasminogen (Plg) | Cell culture; Activity Assays. |
EPB236Hu51 | Eukaryotic Plasminogen (Plg) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB236Hu51 | Active Plasminogen (Plg) | Cell culture; Activity Assays. |
RPB236Hu02 | Recombinant Plasminogen (Plg) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB236Hu07 | Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
PAB236Hu01 | Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
PAB236Hu05 | Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
PAB236Hu02 | Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
LAB236Hu81 | FITC-Linked Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IF. |
LAB236Hu71 | Biotin-Linked Polyclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC. |
MAB236Hu24 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu25 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu26 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu22 | Monoclonal Antibody to Plasminogen (Plg) | WB |
MAB236Hu21 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu23 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu27 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
MAB236Hu29 | Monoclonal Antibody to Plasminogen (Plg) | WB; IHC; ICC; IP. |
SEB236Hu | ELISA Kit for Plasminogen (Plg) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB236Hu | CLIA Kit for Plasminogen (Plg) | Chemiluminescent immunoassay for Antigen Detection. |
LMB236Hu | Multiplex Assay Kit for Plasminogen (Plg) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
KSB236Hu01 | ELISA Kit DIY Materials for Plasminogen (Plg) | Main materials for "Do It (ELISA Kit) Yourself". |