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![Multiplex Assay Kit for Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay)](/upload/static/product/LM.jpg)
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Multiplex Assay Kit for Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay)
GAST; GAS; Gastrin component I; Big gastrin; Gastrin component II; Gastrin component III
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB224Ca
- Organism SpeciesCanis familiaris; Canine (Dog) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 474
US$ 492
US$ 520
US$ 556
US$ 593
US$ 648
US$ 730
Result
For more details, please contact local distributors! US$ 912 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 97-104 | 101 |
EDTA plasma(n=5) | 78-101 | 81 |
heparin plasma(n=5) | 96-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 95-105% | 97-105% | 78-97% | 92-99% |
EDTA plasma(n=5) | 97-105% | 93-102% | 99-105% | 84-99% |
heparin plasma(n=5) | 88-96% | 86-102% | 80-101% | 91-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:GT) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Journal of Diabetes | Omeprazole improves the antiobesity and antidiabetic activity of Exendin-4 in db/db mice. PubMed: 22830490 |
Cell reports | Liver-Derived Systemic Factors Drive b Cell Hyperplasia in Insulin-Resistant States PubMed: PMC3655439 |
Journal of Pharmacy and Pharmacology | Lansoprazole enhances the antidiabetic effect of sitagliptin in mice with diet-induced obesity and healthy human subjects Pubmed: 24628303 |
Pharmacological Reports | Combination of omeprazole with GLP-1 agonist therapy improves insulin sensitivity and antioxidant activity in liver in type 1 diabetic mice Pubmed: 24145087 |
World J Gastroenterol | Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510 |
世界华人消化杂志 | 清醒状态下热射病大鼠胃肠动力与胃泌素、胃动素的变化 Wjgnet:Source |
World J Gastroenterol | Effects of sphincter of Oddi motility on the formation of cholesterol gallstones Pubmed:27350732 |
Nanoscale | Engineering bioinspired bacteria-adhesive clay nanoparticles with a membrane-disruptiveproperty for the treatment of Helicobacter pylori infection. pubmed:27605059 |
Nutrients | The Effect of Salt Intake and Potassium Supplementation on Serum Gastrin Levels in Chinese Adults: A Randomized Trial pubmed:28420122 |
Applied Biological Chemistry | Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3 |
Science of Food and Agriculture | Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer Pubmed: 30479041 |
Journal of tranditional chinese medical sciences | Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome |
Evid Based Complement Alternat Med | The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion Formation Pubmed:35069760 |
Catalog No. | Related products for research use of Canis familiaris; Canine (Dog) Organism species | Applications (RESEARCH USE ONLY!) |
RPB224Ca01 | Recombinant Gastrin (GT) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB224Ca02 | Recombinant Gastrin (GT) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB224Ca01 | Polyclonal Antibody to Gastrin (GT) | WB; IHC; ICC; IP. |
MAB224Ca21 | Monoclonal Antibody to Gastrin (GT) | WB; IHC; ICC; IP. |
CEB224Ca | ELISA Kit for Gastrin (GT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB224Ca | Multiplex Assay Kit for Gastrin (GT) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |