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Multiplex Assay Kit for Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay)
AML3; CBFA1; CCD1; OSF2; PEBP2A; Acute myeloid leukemia 3 ; Core-binding factor alpha-1; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 A
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB011Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 415
US$ 431
US$ 455
US$ 487
US$ 519
US$ 567
US$ 638
Result
For more details, please contact local distributors! US$ 798 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 84-92 | 89 |
EDTA plasma(n=5) | 92-102 | 97 |
heparin plasma(n=5) | 94-105 | 99 |
sodium citrate plasma(n=5) | 90-99 | 96 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-104% | 82-102% | 85-98% | 96-103% |
EDTA plasma(n=5) | 78-96% | 82-104% | 90-104% | 85-93% |
heparin plasma(n=5) | 93-101% | 88-95% | 87-94% | 84-105% |
sodium citrate plasma(n=5) | 83-101% | 94-105% | 80-98% | 93-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:RUNX2) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
International Journal of Cardiology | Valvular osteoclasts in calcification and aortic valve stenosis severity PubMed: 23452891 |
BioMed Research International | Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis Pubmed:27314037 |
Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002 |
International Journal of Pharmaceutical Sciences | MicroRNA-320a protects against osteoarthritis cartilage degeneration by regulating the expressions of BMI-1 and RUNX2 in chondrocytes 10.1691:ph.2017.6890 |
Dental Materials Journal | Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. pubmed:28228629 |
Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995 |
Biochemical and biophysical research communications | Cereblon (CRBN) deletion reverses streptozotocin induced diabetic osteoporosis in mice Pubmed:29353038 |
Journal of Infection and Chemotherapy | Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicity Pubmed:29753615 |
medical science monitor | Silent Mating Type Information Regulation 2 Homolog (SIRT1) Influences Osteogenic Proliferation and Differentiation of MC3T3-E1 Cells via Regulation of miR-132 … Pubmed: 30923307 |
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats Pubmed: 33010420 |
Molecular Medicine Reports | Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism Pubmed: 33000198 |
Effect of Corticopuncture (CP), Photobiomodulation (PBM) and the Combined Method on the Rate of Tooth Movement and Root Resorption: A Molecular, Histological … |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
RPB011Hu01 | Recombinant Runt Related Transcription Factor 2 (RUNX2) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB011Hu01 | Polyclonal Antibody to Runt Related Transcription Factor 2 (RUNX2) | WB; IHC; ICC; IP. |
MAB011Hu22 | Monoclonal Antibody to Runt Related Transcription Factor 2 (RUNX2) | WB; IHC; ICC; IP. |
SEB011Hu | ELISA Kit for Runt Related Transcription Factor 2 (RUNX2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB011Hu | Multiplex Assay Kit for Runt Related Transcription Factor 2 (RUNX2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |