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Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA524Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample Typeplasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.39-400pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.13 pg/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
Result
For more details, please contact local distributors! US$ 821 Add to Price Calculator
Specificity
This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
EDTA plasma(n=5) | 96-105 | 99 |
heparin plasma(n=5) | 78-98 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
EDTA plasma(n=5) | 80-98% | 96-103% | 88-96% | 79-88% |
heparin plasma(n=5) | 80-93% | 78-88% | 88-103% | 91-99% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:TF) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Peptides | Calcitonin gene-related peptide-mediated antihypertensive and anti-platelet effects by rutaecarpine in spontaneously hypertensive rats PubMed: 18625276 |
Canadian Journal of Physiology and Pharmacology | Increased plasma level of asymmetric dimethylarginine in hypertensive rats facilitates platelet aggregation: role of plasma tissue factor Nrcresearchpress: y10-115 |
International immunopharmacology | Ruscogenin attenuates monocrotaline-induced pulmonary hypertension in rats Pubmed: 23538027 |
BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192 |
The Journal of Biological Chemistry | Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during Pregnancy Pubmed:25505239 |
Hippokratia | Thrombosis in Hemodialysis Patients; Their Association with Tissue Factor and Tissue Factor Pathway Inhibitor PubMed: 20011089 |
shock | Noble-Collip Drum Trauma Induces Disseminated Intravascular Coagulation But Not Acute Coagulopathy of Trauma-Shock PubMed: 25423126 |
Perfusion | Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing PubMed: 26243277 |
Molecular Nutrition & Food Research | Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptors PubMed: 25546502 |
Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699 |
Blood Cells, Inflammation and Infection | Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury pubmed:29202212 |
Thrombosis andHaemostasis | Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212 |
Journal of Thrombosis and Haemostasis | Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats Pubmed: 31755229 |
Mediators Inflamm | Amelioration of Coagulation Disorders and Inflammation by Hydrogen-Rich Solution Reduces Intestinal Ischemia/Reperfusion Injury in Rats through NF-κB … Pubmed: 32587471 |
International Journal of Clinical Practice | Effect of Glucocorticoid Administration in Intravenous Pulses on Selected Parameters of the Coagulation System Pubmed:35685509 |
Catalog No. | Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species | Applications (RESEARCH USE ONLY!) |
RPA524Rb02 | Recombinant Tissue Factor (TF) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA524Rb01 | Recombinant Tissue Factor (TF) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA524Rb52 | Polyclonal Antibody to Tissue Factor (TF) | WB; IHC; ICC; IP. |
PAA524Rb51 | Polyclonal Antibody to Tissue Factor (TF) | WB; IHC; ICC; IP. |
PAA524Rb01 | Polyclonal Antibody to Tissue Factor (TF) | WB; IHC; ICC; IP. |
LAA524Rb71 | Biotin-Linked Polyclonal Antibody to Tissue Factor (TF) | WB; IHC; ICC. |
MAA524Rb21 | Monoclonal Antibody to Tissue Factor (TF) | WB; IHC; ICC; IP. |
SEA524Rb | ELISA Kit for Tissue Factor (TF) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA524Rb | Multiplex Assay Kit for Tissue Factor (TF) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA524Rb01 | Antibody Pair for Tissue Factor (TF) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA524Rb01 | ELISA Kit DIY Materials for Tissue Factor (TF) | Main materials for "Do It (ELISA Kit) Yourself". |