≡

Multiplex Assay Kit for Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay)

CALCA; CALC1; KC; CCP; PDN-21; Katacalcin; Calcitonin carboxyl-terminal peptide

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 82-92 88
heparin plasma(n=5) 93-104 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-104% 78-103% 86-101% 85-103%
EDTA plasma(n=5) 92-101% 97-105% 86-96% 89-97%
heparin plasma(n=5) 91-98% 79-104% 78-92% 82-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CT) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
indian Journal of Experimental Biology Effect of consumption of fatty acids, calcium, Vitamin D and boron with regular physical activity on bone mechanical properties and corresponding metabolic hormones in rats Niscair: 13737
Toxicol Ind Health The effect of supplementation of calcium, vitamin D, boron, and increased fluoride intake on bone mechanical properties and metabolic hormones in rat PubMed: 22782709
The Journal of Clinical Endocrinology & Metabolism Interleukin-2 and Lanreotide in the Treatment of Medullary Thyroid Cancer: In Vitro and In Vivo Studies Pubmed: 23884781
Academic Journal Effect of whole body vibration on healthy rat plasma parameters Ebscohost: Source
Indian Journal of Experimental Biology Effect of consumption of fatty acids, nutrients and regular physical activity on bone mechanical properties in rats Pubmed:22439438
International journal of cardiology Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies. pubmed:27448535
7 Cell-matrix signals specify bone endothelial cells during developmental osteogenesis. pubmed:28218908
International Journal of Advanced Research in Biological Sciences Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 10.22192/ijarbs.2017.04.04.018
cell proliferation p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway Pubmed: 32215984
Nature Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia Pubmed: 33149301
Clin Chim Acta Copeptin in fluid disorders and stress Pubmed:35143773
Catalog No. Related products for research use of Canis familiaris; Canine (Dog) Organism species Applications (RESEARCH USE ONLY!)
RPA472Ca01 Recombinant Calcitonin (CT) Positive Control; Immunogen; SDS-PAGE; WB.
PAA472Ca01 Polyclonal Antibody to Calcitonin (CT) WB; IHC; ICC; IP.
MAA472Ca21 Monoclonal Antibody to Calcitonin (CT) WB; IHC; ICC; IP.
CEA472Ca ELISA Kit for Calcitonin (CT) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA472Ca Multiplex Assay Kit for Calcitonin (CT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.