Multiplex Assay Kit for Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay)

Neurotrophin; Abrineurin

(Note: Up to 8-plex in one testing reaction)

  • Product No.LMA011Mv
  • Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Same name, Different species.
  • Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length3.5h
  • Detection Range0.01-10ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
  • DownloadInstruction Manual
  • UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
  • FOB US$ 389  US$ 404  US$ 426  US$ 456  US$ 486  US$ 531  US$ 598  US$ 748  Add to Price Calculator Result
    For more details, please contact local distributors!

Specificity

This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-105 101
EDTA plasma(n=5) 85-103 90
heparin plasma(n=5) 91-99 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-104% 95-102% 90-99% 80-92%
EDTA plasma(n=5) 88-97% 79-105% 95-102% 87-101%
heparin plasma(n=5) 80-99% 86-94% 80-103% 87-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:BDNF) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
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International Conference on Bioscience Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative Damage Psrcentre: 212059
Neuroscience Letters Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone mice ScienceDirect: S0304394010015788
BioMed Research International Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord. NCBI: PMC3925622
Behavioural Brain Research The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factor Pubmed:25446810
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Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats pmc:PMC4812310
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064
The International Journal af Applied Research in Veterinary Medicine Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
J Chem Neuroanat. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. pubmed:27914953
Int Immunopharmacol. PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice. pubmed:27940378
The Journal of Maternal-Fetal & Neonatal Medicine Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639
IOS Press Content Library An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease
Bipolar Disorders Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic … bdi.12449
Elsevier Associations of blood parameters with age, feed efficiency and sampling routine in young beef bulls science:article
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American Journal of Translational Research A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979
European Journal of Pharmacology Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal. pubmed:29042206
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Nutrients Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Pubmed:29890692
Neurotoxicity Research Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley Rats Pubmed:28933048
Neurotoxicology Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats Pubmed:29522778
Neuroscience Letters Long term Westernized diet leads to region-specific changes in brain signaling mechanisms Pubmed:29655945
Applied Physiology Nutrition and Metabolism Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclists Pubmed: 30286300
Heliyon Establishment and evaluation of a novel mouse model of peri/postmenopausal depression Doi: 10.1016/j.heliyon.2019.e01195
BioMed Research International Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression Pubmed: 30834799
American Journal of Emergency Medicine Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute stroke Pubmed: 30826210
Neurochemistry International Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in mice Pubmed: 31077758
REVISTA DA ASSOCIACAO MEDICA BRASILEIRA Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with Depression Pubmed: 30994834
Journal of Alzheimer's Disease Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model Mice Pubmed: 31561359
Biological Trace Element Research Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain Signaling Pubmed: 31797207
Psychopharmacology Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats Pubmed: 32088835
Experimental and Therapeutic Medicine Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Pubmed: 32010279
Journal of Critical Care Association of neuronal repair biomarkers with delirium among survivors of critical illness Pubmed: 31896448
Journal of Clinical Neuroscience CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic children Pubmed: 32327377
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Neurotoxicology Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice Pubmed: 32522471
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Metab Brain Dis Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men 34762211
Transl Psychiatry Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression 34824208
Iranian Journal of Basic Medical Sciences Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons 34712429
J Sex Med Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid … 34384732
Cell Biochem Funct CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway 34296452
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader … Pubmed:35098494
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Catalog No. Related products for research use of Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
LMA011Mv Multiplex Assay Kit for Brain Derived Neurotrophic Factor (BDNF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.