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Biotin-Linked Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)

  • Product No.LAP549Mu71
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceAntibody labeling
  • Ig Type IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration500µg/mL
  • Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
  • ApplicationsWB; IHC; ICC.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 131  US$ 305  US$ 436  US$ 1090  US$ 4360 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against WNT5A. It has been selected for its ability to recognize WNT5A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Liver International Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis Pubmed:25488180
Journal of Physiology and Biochemistry Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells PubMed: 26547443
Liver Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis PubMed: 25488180
Transl Res Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures PubMed: 26163991
不确定杂志名 Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … servlets:MCRFileNodeServlet
Life Sciences Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618
Arteriosclerosis, Thrombosis, and Vascular Biology  Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … Pubmed:29674475
Stem Cells Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells Pubmed:29377497
Science Translational Medicine Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases Pubmed: 31534019
J Clin Endocrinol Metab Role of wnt5a in Metabolic Inflammation in Humans Pubmed: 30137542
Cancer Research Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis Pubmed: 31932454
CLINICAL BIOCHEMISTRY Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease Pubmed: 33188773
Sao Paulo Medical Journal Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … Pubmed: 32491086
Biomaterials An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470
J Clin Periodontol Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study Pubmed:35569034
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