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Biotin-Linked Polyclonal Antibody to Defensin Alpha 5, Paneth Cell Specific (DEFa5)

DEF-A5; DEF5; HD-5

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DEFa5. It has been selected for its ability to recognize DEFa5 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Vaccine On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment Pubmed:25444792
Differentiating Asymptomatic Bacteriuria From Urinary Tract Infection in the Pediatric Neurogenic Bladder Population: NGAL As a Promising Biomarker Pubmed: 31548788
Inflamm Bowel Dis Antagonism of Adherent Invasive E. coli LF82 With Human ¦Á-defensin 5 in the Follicle-associated Epithelium of Patients With Ileal Crohn's Disease 33336693
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