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Western Blot; Sample: Rat Cerebrum lysate
Primary Ab: 0.2μg/ml Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12)
Second Ab: 0.2μg/ml HRP-Labeled Streptavidin (Catalog: IS089)
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Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 12 (MMP12)
MME; HME; ME; Macrophage Metalloelastase; Macrophage Elastase
- Product No.LAA402Ra71
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourceAntibody labeling
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibody n/a
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies. - Downloadn/a
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 113
US$ 263
US$ 376
US$ 940
US$ 3760
For more details, please contact local distributors!
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MMP12. It has been selected for its ability to recognize MMP12 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| International journal of colorectal disease | Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repair Pubmed: 23619615 |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655 |
| American Journal of Physiology - Lung Cellular and Molecular Physiology | Inspiratory resistive breathing induces MMP-9 and MMP-12 expression in the lung Pubmed:25595645 |
| Photodermatol Photoimmunol Photomed. | Protective effects of Aloe sterols against UVB‐induced photoaging in hairless mice pubmed:27995657 |
| Яковлева Наталья Юрьевна | |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats | |
| Nat Commun | Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice Pubmed:35031603 |