Biotin-Linked Monoclonal Antibody to Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- Product No.LAA071Eq76
- Organism SpeciesEquus caballus; Equine (Horse) Same name, Different species.
- SourceAntibody labeling
- Ig Isotype IgG
- PurificationProtein A + Protein G affinity chromatography
- LabelBiotin
- Original Antibody MAA071Eq26-Monoclonal Antibody to Interleukin 1 Alpha (IL1a)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 135
For more details, please contact local distributors! US$ 314 US$ 449 US$ 1123 US$ 4490
SPECIFITY
The antibody is a mouse monoclonal antibody raised against IL1a. It has been selected for its ability to recognize IL1a in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- Protein A/G Purification Column
- Staining Solution for Cells and Tissue
- Positive Control for Antibody
- Tissue/Sections Customized Service
- Phosphorylated Antibody Customized Service
- Western Blot (WB) Experiment Service
- Immunohistochemistry (IHC) Experiment Service
- Immunocytochemistry (ICC) Experiment Service
- Flow Cytometry (FCM) Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Immunofluorescence (IF) Experiment Service
- Buffer
- DAB Chromogen Kit
- SABC Kit
- Real Time PCR Experimental Service
Magazine | Citations |
The Veterinary Journal | Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy Pubmed: 24662024 |
Int J Clin Exp Pathol | Expression of S100 family proteins in neonatal rats with sepsis and its significance PubMed: 25973048 |
Tumor Biology | Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151 |
Parasites & Vectors | Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced highlevels of humoral responses and pro-inflammatory cytokines but confers limited protection. pubmed:27502394 |
Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy pubmed:28333148 |
Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349 |
Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets pubmed:28811665 |
Arthritis Research and Therapy | Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. pubmed:29191221 |
International Journal of Molecular Sciences | Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus Pubmed:30066828 |
Journal of Molecular Neuroscience | Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation Pubmed:29243061 |
Journal of Maternal-Fetal & Neonatal Medicine | Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage Pubmed:29357727 |
Brazilian Dental Journal | Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems Pubmed: 30970054 |
The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency | |
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus |
Polish Journal of Veterinary Sciences | Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein … Pubmed: 31560476 |
Nanomedicine | The toxicity of alumina nanoparticles to immune system in mice Pubmed: 32162999 |
Theranostics | Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration Pubmed: 32308770 |
STEM CELLS AND DEVELOPMENT | IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways Pubmed: 33107399 |
FASEB JOURNAL | ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway Pubmed: 32908567 |
BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225 |
Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis 34256094 |
Brain Res | Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells 34780748 |
Animals (Basel) | Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation 34944269 |
Animals (Basel) | Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation … Pubmed:34944269 |