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Eukaryotic Collagen Type I (COL1)

  • Product No.EPA571Eq61
  • Organism SpeciesEquus caballus; Equine (Horse) Same name, Different species.
  • SourceEukaryotic expression
  • Host293F cell
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass57.7kDa
  • Accurate Molecular Mass60kDa(Analysis of differences refer to the manual)
  • Residues & TagsArg1226~Phe1462 with N-terminal His Tag and C-terminal Fc Region of Human IgG1
  • Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point5.4
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 236  US$ 590  US$ 1180  US$ 3540  US$ 8850 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of hypertension Urotensin II induces phenotypic differentiation, migration, and collagen synthesis of adventitial fibroblasts from rat aorta PubMed: 18475149
Cardiovascular Research Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway OxfordJournals: source
Acta Otolaryngol Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa PubMed: 19958244
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
Connect Tissue Res. Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans Pubmed: 23758268
Bulletin of Experimental Biology and Medicine Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty Pubmed: 24319714
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
European Archives of Oto-Rhino-Laryngology Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats Pubmed: 24077847
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818
J Physiol Pharmacol. Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet Pubmed: 23568967
22 Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses ScienceDirect: S0142961214003135
Journal of Clinical and Experimental Hepatology Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis ScienceDirect: S0973688314000073
Human Molecular Genetics Online Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10 Pubmed:24659498
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Journal of Biomedical Materials Research Part A Carboxymethyl cellulose—hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications Pubmed:23720392
Experimental Biology adn Medicine Endostar, a novel human recombinant endostatin, attenuates liver fibrosis in CCl4-induced mice Pubmed:24872431
International Journal of Clinical &amp; Experimental Pathology Mast cell chymase in keloid induces profibrotic response via transforming growth factor-β1/Smad activation in keloid fibroblasts Pubmed:25120737
FEBS Letters Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352
Cardiology KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload Pubmed:25633973
PLOS ONE Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells Pubmed:25635832
Journal of Colloid and Interface Science Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses Pubmed:25706198
Macromolecular Bioscience Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells PubMed: 26126665
journal of hard Tissue Biology Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite Article: Jhtb
Mol Med Rep NIM811 downregulates transforming growth factor PubMed: 26573209
Front Pharmacol Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats PubMed: 26483688
Chemosphere Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue PubMed: 25655816
J Surg Res Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition PubMed: 25911951
Molecular and Cellular Biochemistry SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis PubMed: 26112906
Biointerphases Incorporation of nanostructured hydroxyapatite and poly(N-isopropylacrylamide) in demineralized bone matrix enhances osteoblast and human mesenchymal stem cell activity PubMed: 26443012
Gels On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery 2310-2861: 1
Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470
Int J Clin Exp Med Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506
International Journal of Molecular Sciences Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration Pubmed:27092492
中国眼耳鼻喉科杂志 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响 article:11593
Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470
scientific reports IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy Pubmed:27263531
Cell Mol Immunol  Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica Pubmed:27157496
Кардіологія Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти bitstream:123456789
Inflammation Research Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy pubmed:27516211
Indian Journal of Pharmaceutical Education and Research Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-β1 sites:default
Heart & Vessels Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR pubmed:28004175
biochemical and biophysical research communications Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. pubmed:28153731
Frontiers in Pharmacology Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-β1/Smad-mediated epithelial to mesenchymal … PMC5108931
International Journal of Molecular Sciences Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study. pubmed:27556453
BMC Cancer Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108
J Cell Physiol. An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. pubmed:28240358
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440
Acta Biomater. Antiresorptive and anti-angiogenetic octacalcium phosphate functionalized with bisphosphonates: An in vitro tri-culture study. pubmed:28238916
The American Journal of Chinese Medicine Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747
Biomedical and Environmental Sciences Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo pubmed:29081339
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111
Dental Materials Journal Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. pubmed:28228629
Biomedicine & pharmacotherapy Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995
Journal of Applied Biomaterials & Functional Materials Demineralized dentin and enamel matrices as suitable substrates for bone regeneration pubmed:28731486
Acta Biomaterialia Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation pubmed:28698163
Advanced Healthcare Materials Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. pubmed:29120858
Macromolecular Bioscience Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response Pubmed:29877029
International Journal of Molecular Medicine Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced … Pubmed:29620144
PLoS One Effect of hyperbaric oxygen therapy (HBO) on implant-associated osteitis in a femur fracture model in mice Pubmed:29377928
Royal Society of Chemistry In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment c8ra00768c
European Food Research and Technology Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis 10.1007:s00217-018-3119-z
Journal of Applied Biomaterials & Functional Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization Pubmed:30045659
Theriogenology Effect of transforming growth factor-β1 on α-smooth muscle actin and collagen expression in equine endometrial fibroblasts Pubmed: 30321755
ACS Applied Materials & Interfaces Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications Pubmed: 30433758
Chemico-Biological Interactions Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells Pubmed: 30731080
Oxidative Medicine and Cellular Longevity Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway
Tropical Journal of Pharmaceutical Research Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.
Scientific Reports Low fibrosis biomarker levels predict cardiac resynchronization therapy response Pubmed: 30988339
Experimental Lung Research High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects Pubmed: 31290711
BMC Nephrology Serum sclerostin levels are positively related to bone mineral density in peritoneal dialysis patients: a cross-sectional study Pubmed: 31315601
Archives of Dermatological Research Extracellular matrix deposition by adipose-derived stem cells and fibroblasts: a comparative study Pubmed: 31616972
Drug Delivery and Translational Research A multifunctional dexamethasone-delivery implant fabricated using atmospheric plasma and its effects on apoptosis, osteogenesis and inflammation Pubmed: 31898081
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs Pubmed: 32146192
Molecular Metabolism SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α Pubmed: 32029232
ACS Applied Materials & Interfaces ZIF-8 Modified Multifunctional Bone-Adhesive Hydrogels Promoting Angiogenesis and Osteogenesis for Bone Regeneration Pubmed: 32814397
in vitro cellular & developmental biology-animal Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix Pubmed: 32588253
Molecular Medicine Circulating fibrocytes traffic to the lung in murine acute lung injury and predict outcomes in human acute respiratory distress syndrome: a pilot study Pubmed: 32460694
Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда
Ann Med Surg Trauma magnitude of the meta-epyphyseal cancellous affects the incidence of deep vein thrombosis. A prospective cohort study on the dynamic of collagen I ¡­ 33717478
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Uncarboxylated osteocalcin reverses the high glucose?induced inhibition of the osteogenic differentiation of MC3T3E1 cells via the GPRC6A/cAMP/PKA/AMPK signaling pathway 33786607
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
Russian Journal of Cardiology Early diagnosis of myocardial fibrosis in patients with epicardial obesity
Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction
Parasit Vectors Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049
Pharmacol Res Esomeprazole Alleviates Fibrosis in Systemic Sclerosis by Modulating AhR/Smad2/3 Signaling Pubmed:34995795
Catalog No. Related products for research use of Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
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