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Rat Model for Embolic Cerebral Infarction (ECI)

ECI

  • Product No.DSI681Ra04
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceSmall emboli occlusion
  • Model Animal StrainsSD rats, healthy, male, body weight 180g~200g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
  • Modeling Method"Thrombosis production: SD rats, intraperitoneal injection of chloral hydrate (350 ~ 400 mg/kg body weight dose) or pentobarbital sodium (50 ~ 60 mg/kg body weight dose), the rats were fixed in supine position on the operating table, cut off the operation area of hair, skin incision and conventional disinfection, left lower extremity skin, find the femoral vein puncture and venous blood of 0.5 ml with 0.15 ml thrombin (200 U plus 0.4 ml saline) immediately after mixing, then injected 2 continuous epidural anesthesia catheter, standing for 15 ~ 20 min. after skin suture, catheter blood clots within the syringe slowly into the saline, cleaning 2 ~ 3 times to remove thrombus surface of red blood cells. Under the microscope, select the cellulose rich thrombus was cut into 14 ~ 18 2 mm long segments, then the phosphate buffer was selected these 7.4 segments on the pH (or saline). In phosphate buffer static The 4 ~ 5 h, the complete condensation, together with phosphate buffer with thrombus will be inhaled a transparent continuous epidural anesthesia catheter catheter in standby, pay attention to avoid the inhalation of air.
    The rat cerebral embolism model : to be condensed thrombosis after 4h, the rats were anesthetized again, operation skin disinfection. Jing Zhengzhong left the skin incision, blunt isolated left CCA, ICA and ECA, 2 branch ligation of ECA, in 2 lines in ECA, ECA distal end ligation ligation of the ICA branch. Pterygopalatine artery occluded for CCA and ICA, ECA in the distal cut a ""V"" incision, No. 22 trocar by ECA distal to the proximal cannula. The needle core is drawn out after intubation, the inserted sleeve is placed in the CCA bifurcation for fixed line casing, a transparent catheter containing thrombus No. 20 No. 22 trocar core is connected to the casing, loosen the internal carotid artery clamp, slowly catheter thrombosis and injection of ICA phosphate buffer (30 S) after pulling out casing. Ligation of the proximal end of ECA, loosen the carotid artery clamp, skin suture. keep the body temperature during the operation process, keep the rectal temperature in 36 ~ 37 degrees."
  • Applicationsn/a
  • Downloadn/a
  • UOM Each case
  • FOB US$  
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Model Evaluation

Pathological Results

Cytokines Level

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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