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Mouse Model for Pulmonary Embolism (PE)

  • Product No.DSI517Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceThrombin induced
  • Model Animal StrainsBalb/c mice (SPF class), male, weeks in age:4weeks~6weeks, 20g~22g.
  • Modeling GroupingRandomly divided into groups: Control group, Model group, Positive drug group and Test drug group, 15 mice per group.
  • Modeling Period1~2 days
  • Modeling Method1. 22g-26g mice, with 3% Pentobarbital Sodium, with 80mg/kg intraperitoneally anesthetized mice, mice in supine position, the limbs and head with tape fixed on anatomical plate, with 8% sodium sulfide to remove the neck hair, povidone iodine disinfection in preparation for surgery.
    2. Incision on the middle of the neck, the separation of soft tissue and muscle, exposure to the left side of the jugular vein, inject
    Thrombin solution (soluble in 0.9% normal saline, dose 20~75U/kg) to the internal jugular vein .
    3. Clean up the operation field, suture the neck incision, stay awake and put mice back to clean cage, observe the state and the state of the mouse and record.
    4. Sham operation is the same as that operation in modeling group,but the normal saline injected into the jugular vein.
    5. After 24hrs, kill the mice, take the left lung tissue, 4% poly formaldehyde solution fixed, paraffin embedded; the rest of the lung tissue -80 degree preservation.
  • ApplicationsDisease Model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 140 
    For more details, please contact local distributors!

Model Evaluation

Peripheral blood platelet count:
collect 20ul mice peripheral blood , put in 380 μL (10g/L) ammonium oxalate dilution, after mixing and use blood cell counting plate platelet count.

Pathological Results

Take the left lung, 4% poly formaldehyde fixed, and then dehydration, paraffin embedded, sliced (thickness of 5μm), HE staining.
A few hours after that alveolar exudation and hemorrhage, alveolar septal thickening, which a large number of inflammatory cell infiltration, visible large artery embolism, platelets and red blood cell aggregation and adhesion to around the embolus.

Cytokines Level

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
DSI517Mu01 Mouse Model for Pulmonary Embolism (PE) Disease Model
TSI517Mu07 Mouse Lung Tissue of Pulmonary Embolism (PE) Paraffin slides for pathologic research: IHC,IF and HE,Masson and other stainings