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Rat Model for Atherosclerosis (ASVD)

Arteriosclerotic Vascular Disease

  • Product No.DSI512Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceInduced by “high fat diet + vitamin D3 +passive smoking intervention(optional)"
  • Model Animal StrainsSD rats(SPF), healthy, gender in half, 4 weeks, bodyweight:180~220g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses),n=15.
  • Modeling Period13w
  • Modeling Method1.SD rats were randomly divided into 6 groups, each group of 15, raised in the SPF class animal room, the room to maintain a constant temperature (22℃±1℃) and alternating light and dark. Rats were allowed to drink water freely.
    2. From the 2nd week,the rats in the blank control group were fed with normal diet until the end of the thirteenth week.
    The model group rats were given “high fat diet + vitamin D3 + intraperitoneal injection of passive smoking intervention(optional)”;
    high fat diet: 4% cholesterol, 0.5% sodium cholate, 0.2% propylthiouracil, 5% sugar, 10% lard, 80.3% basic diet; and with intraperitoneal injection of vitamin D3(VD3), according to a total dose of 900,000 U/kg, given in 4 times: 600,000IU/kg was administered at the beginning of the 2nd week,100,000 IU/kg at the beginning of the 4th,7th and 10th week by intraperitoneal injection.
    3.Along the ventral midline to cut the skin and subcutaneous layers, and open the chest, take the blood in the left ventricular apex position to get about 2-3ml blood, and separation of serum.
    4.After collecting blood,careful separation of aorta, thoracic aorta, ascending aorta root separation of aorta to aorta, collecting blood vessels from the aortic root upwards(a length of about 2cm), in 0.9% sodium chloride in the gently rinsed 2 times and then put into 4% fixed neutral Formaldehyde Solution, fixed 24 hours before pathology detection.
  • ApplicationsDisease Model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 320 
    For more details, please contact local distributors!

Model Evaluation

1. Serum total cholesterol(TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) concentration was measured by automatic biochemical analyzer. After the assay, the serum was kept in the refrigerator at -20℃.
The level of serum total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) in the model group was significantly higher than that in the control group.
2. In the normal control group, the intima of the normal control group was white, the surface was smooth, and there was no lipid plaque formation; In the model group, the intima of aorta was rough and thickened, and the plaque was protruding into the lumen.

Pathological Results

3. HE staining, normal control group rat aortic vascular intimal smooth, endothelial integrity, endometrium and the middle cell arrangement rules, clear cell outline, no foam cell formation; the model group showed that vascular wall is not smooth, endometrium is not continuous, wrinkled. The proliferation of the middle membrane was obvious, the cell morphology was diverse, the arrangement was disordered and loose, and the gap was widened.
4. Immunohistochemical detection of thoracic aorta smooth muscle actin: α-actin antibody of smooth muscle antibody was characterized by specific smooth muscle antibody. It can be seen that the normal control group and the high fat group were normal, and the intima of the high lipid + vitamin D3 group had thicker α-actin positive. The surface of the high fat ten vitamin D3 group had only a very thin α-actin positive.

Cytokines Level

The content of inflammatory index MDA in serum was determined by MDA kit.The level of MDA in the model group was significantly higher than that in the control group.

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
DSI512Ra01 Rat Model for Atherosclerosis (ASVD) Disease Model