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Rat Model for Hypertension (HT)

High Blood Pressure

  • Product No.DSI511Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Prototype SpeciesHuman
  • Sourceinduced by Two-kidney one-clip（2K1C）method
  • Model Animal StrainsWistar rats(SPF), healthy, age:3~4w, half gender, body weight:180g~200g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses), n=15.
  • Modeling Period8w
  • Modeling Method1. Hypertension model by high salt diet.
    The control group was given basic feed, and the model group was fed with high salt diet (4% NaCl), lasted for 8 weeks.
    or
    2. Two-kidney one-clip （Gloidblatt 2K1C ）renovascular hypertensive model:
    Chloral hydrate anesthetized rats, carefully isolated renal artery, placed standard wire in the renal artery independent artery bifurcation (the diameter = 0.2mm), silver clips close the clip, and then remove the standard wire, suture muscle, skin layer by layer. The control group was not placed without standard wire, do not close the clip, other operations are the same.
    Sample Collection:
    1. Plasma collection: After 8 weeks, rats were anesthetized , collect 2ml blood from the inferior vena cava, stored at -80℃.
    2. Acquisition of the heart and aorta: rats were anesthetized, taking the heart, carefully and rapidly separating the aorta from the aortic arch to the branch of the iliac artery under a microscope. Heart and aorta were placed on 10% neutral formalin solution.
  • ApplicationsDisease Model
  • Downloadn/a
  • UOM Each case
  • FOB US$ 200 
    For more details, please contact local distributors!

Model Evaluation

1. Continuous measure the blood pressure for 3 times, taking the average value as a measurement value, once a week. Systolic blood pressure of 85~120mm Hg (1mm Hg=0.133 kPa) in normotensive rats was selected in the present study. After modeling, the blood pressure was measured regularly. When the systolic blood pressure of rats was higher than 20mm Hg before operation, it was determined to be a successful model of hypertension. The blood pressure of model group was significantly higher than that of control group.

Pathological Results

After embedding the heart and aorta, the slices were 3um thick and stained by HE. Compared with the control group, model group rats showed cardiomyocyte hypertrophy and necrosis, myocardial fiber breakage, muscle gap were widened significantly, more macrophages and lymphocytes, fibroblast infiltration were observed in fracture and necrosis area, myocardial collagen fibers increased significantly are resulted in the left ventricular remodeling in rats. Compared with the control group, the vascular wall were thickening, vascular smooth muscle cell were proliferation or hypertrophy, the lumen diameter were decreased.

Cytokines Level

Assay of Hcy, MDA, Ang II, ET-1 and GSH-Px, Renin in plasma:
The model group of plasma homocysteine in hypertensive rats (Hcy), malondialdehyde (MDA), angiotensin II (Ang II), endothelin 1 (ET-1) content and glutathione peroxidase (GSH-Px), renin activity (Renin) were higher than the control group.

Statistical Analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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