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Multiplex Assay Kit for Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array)

SIR2L4; Silent Mating Type Information Regulation 2 Homolog 4; SIR2-like protein 4; NAD-dependent protein deacetylase sirtuin-4

Specificity

This assay has high sensitivity and excellent specificity for detection of Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-102 98
EDTA plasma(n=5) 90-99 95
heparin plasma(n=5) 84-105 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 80-101% 91-98% 78-92%
EDTA plasma(n=5) 95-103% 79-103% 87-98% 81-96%
heparin plasma(n=5) 98-105% 88-102% 84-93% 79-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Growth Preliminary data on the relationship between circulating levels of Sirtuin 4, anthropometric and metabolic parameters in obese subjects according to growth hormone/insulin-like growth factor-1 status PubMed: 25466907
hindawi ADHERENCE TO THE MEDITERRANEAN DIET AND CIRCULATING LEVELS OF SIRTUIN 4 IN OBESE 6101254.pdf
Oxidative Medicine and Cellular Longevity Adherence to the Mediterranean Diet and Circulating Levels of Sirtuin 4 in Obese Patients: A Novel Association pubmed:28706576
Elife SIRT2 deacetylase regulates the activity of GSK3 isoforms independent of inhibitory phosphorylation Pubmed:29504933
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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SEE914Hu ELISA Kit for Sirtuin 4 (SIRT4) Enzyme-linked immunosorbent assay for Antigen Detection.
LME914Hu Multiplex Assay Kit for Sirtuin 4 (SIRT4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CME914Hu Multiplex Assay Kit for Sirtuin 4 (SIRT4) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.