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Multiplex Assay Kit for Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array)
FTH1; FTHL6; PIG15; PLIF; Ferritin Heavy Chain; Apoferritin; Placenta Immunoregulatory Factor; Proliferation-Inducing Protein 15; Cell proliferation-inducing gene 15 protein
- Product No.CMD021Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range1.9531-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.651pg/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 588
US$ 840
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 85-101 | 96 |
| EDTA plasma(n=5) | 87-102 | 94 |
| heparin plasma(n=5) | 81-95 | 90 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ferritin, Heavy Polypeptide (FTH) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-93% | 90-104% | 86-101% | 85-105% |
| EDTA plasma(n=5) | 80-95% | 81-101% | 95-102% | 89-97% |
| heparin plasma(n=5) | 78-97% | 81-102% | 82-97% | 92-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| PLoS One | Presence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical One PubMed: PMC3765839 |
| Am J Physiol Lung Cell Mol Physiol. | Methemoglobin-induced signaling and chemokine responses in human alveolar epithelial cells Pubmed:24142518 |
| J Alzheimers Dis | The Oral Iron Chelator, Deferasirox, Reverses the Age-Dependent Alterations in Iron and Amyloid-β Homeostasis in Rat Brain: Implications in the Therapy of Alzheimer’s Disease PubMed: 26484920 |
| Eur Radiol | MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter Pubmed:26960542 |
| Exp Hematol. | Impact of TET2 deficiency on iron metabolism in erythroblasts. pubmed:28167288 |