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Multiplex Assay Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array)

CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine

Specificity

This assay has high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-101 96
EDTA plasma(n=5) 78-92 82
heparin plasma(n=5) 81-103 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-97% 98-105% 84-92% 97-105%
EDTA plasma(n=5) 78-105% 84-92% 98-105% 82-99%
heparin plasma(n=5) 82-103% 83-96% 83-103% 89-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Medicine (Baltimore). Increased Serum Levels of Macrophage Inflammatory Protein-3α and Cystatin A Predict a Poor Prognosis of Nasopharyngeal Carcinoma Pubmed:25396333
Cancer Serum chemokine network correlates with chemotherapy in non-small cell lung cancer PubMed: 25976768
American Journal of Cancer Research CCR6+ B lymphocytes responding to tumor cell-derived CCL20 support hepatocellular carcinoma progression via enhancing angiogenesis. pubmed:28560063
Biological Research In vitro chemokine (CC motif) receptor 6-dependent non-inflammatory chemotaxis during spermatogenesis 10.1186:s40659-018-0161-z
BioMed Research International Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy
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SEA095Po ELISA Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA095Po Multiplex Assay Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMA095Po Multiplex Assay Kit for Macrophage Inflammatory Protein 3 Alpha (MIP3a) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.