Packages (Simulation)

Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody

Image (I)

DAB staining on IHC-P; Sample: Porcine Cerebrum Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Porcine Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human ERK1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Image (II)

Western Blot; Sample: Lane1: MCF7 cell lysate; Lane2: 293T cell lysate Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Liver lysate; Lane4: Mouse Cerebrum lysate; Lane5: Mouse Heart lysate; Lane6: Porcine Liver lysate; Lane7: Porcine Cerebrum lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Caprine Liver lysate; Lane2: Caprine Cerebrum lysate; Lane3: Caprine Heart lysate; Lane4: Canine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Cavia Cerebrum lysate; Lane7: Equine Heart lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Anti-Extracellular-Signal-Regulated-Kinase-1-(ERK1)-Polyclonal-Antibody-CAB357Hu01.pdf

Certificate

Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

Product No.CAB357Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration500µg/mL
Organism Species MoreMus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
ApplicationsLoading Control of WB.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 107 251 US$ 251 US$ 358 US$ 895 US$ 3580
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine	Citations
Graefe's Archive for Clinical and Experimental Ophthalmology	Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909
International Journal of Reproductive Medicine	Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317
Sci Rep	Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
RPB357Hu01	Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)	Positive Control; Immunogen; SDS-PAGE; WB.
PAB357Hu01	Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
CAB357Hu01	Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody	Loading Control of WB.
MAB357Hu22	Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
MAB357Hu21	Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)	WB; IHC; ICC; IP.
CAB357Hu22	Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Monoclonal Antibody	Loading Control of WB.
SEB357Hu	ELISA Kit for Extracellular Signal Regulated Kinase 1 (ERK1)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB357Hu	Multiplex Assay Kit for Extracellular Signal Regulated Kinase 1 (ERK1) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.