Active Luteinizing Hormone (LH)
ICSH; Lutropin; Interstitial Cell Stimulating Hormone
- Product No.APA441Hu62
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.05% SKL.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point8.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 320
For more details, please contact local distributors! US$ 800 US$ 1600 US$ 4800 US$ 12000
ACTIVITY TEST
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Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor. LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol. A functional binding ELISA assay was conducted to detect the interaction of recombinant human CGa/LHb and recombinant human Dopamine Receptor D1 (DRD1). Briefly, biotin-linked CGa/LHb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to DRD1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of CGa/LHb and DRD1 was shown in Figure 1, the EC50 for this effect is 0.34 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine | Citations |
Molecular and Cellular Endocrinology | Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes. PubMed: 19686804 |
International Journal of Clinical and Experimental Medicine | Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004 |
Biochemical Journal | PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone PubMed: 21108604 |
Molecular and Cellular Endocrinology | Effects of genistein and estrogen receptor subtype-specific agonists in ArKO mice following different administration routes ScienceDirect: S0303720709004043 |
Asian Journal of Andrology | Brief maternal exposure of rats to the xenobiotics dibutyl phthalate or diethylstilbestrol alters adult-type Leydig cell development in male offspring PubMed: 23314658 |
EXCLI Journal | Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Excli: Source |
International Journal of Molecular and Cellular Medicine | Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777 |
Reproductive Toxicology | Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines Pubmed: 24486453 |
Urologia internationalis | Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982 |
Journal of Materials Chemistry B | Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering Rsc: Source |
Electromagnetic Biology and Medicine | The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source |
Academic Sciences | Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source |
Biochemical and Biophysical Research Communications | Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis ScienceDirect: S0006291X14001843 |
Reproductive Toxicology | Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring Pubmed:24126190 |
Mol Cell Endocrinol. | Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Pubmed:24316378 |
Andrologia | Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220 |
Environmental Research | Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141 |
Genet Mol Biol. | Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846 |
Turkish Journal of Urology | Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source |
Andrologia | Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis PubMed: 24576220 |
European Journal of Pharmacology | The effect of R-(-)-deprenyl administration on reproductive parameters of rat males PubMed: 25725114 |
Andrologia | Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866 |
Reprod Toxicol | Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359 |
Biology of Reproduction | Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114 |
Andrologia | Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866 |
Int J Mol Sci. | Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice Pubmed:26771609 |
PLoS Genet. | A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis Pubmed:26977770 |
Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373 |
33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389 |
environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061 |
Toxicological Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring pubmed:27803384 |
Andrologia | Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943 |
Biology of Reproduction | Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114 |
J Endocrinol. | Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054 |
Int J Pharm. | New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269 |
Environmental Science & Technology | Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686 |
Journal of Medicinal Chemistry | Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147 |
Society of Toxicology | Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells pubmed:29149324 |
Reproductive Toxicology | Vitamin D deficiency impairs testicular development and spermatogenesis in mice pubmed:28655646 |
The Journal of Biological Chemistry. | Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. pubmed:28578315 |
The Journal of Toxicological Sciences | High sensitivity of testicular function to titanium nanoparticles pubmed:28496042 |
Journal of Orthopaedic Research | The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779 |
Anticancer Research | Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy pubmed:29187457 |
DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999 |
Reproduction. | Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593 |
Archives of Toxicology | Atorvastatin decreases steroid production in H295R cells and in major endocrine tissues of male rats Pubmed:29574565 |
FASEB Journal | Vasorin: a newly identified regulator of ovarian folliculogenesis Pubmed:29259033 |
Stem Cell Research & Therapy | The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657 |
Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625 |
Toxicological Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons 10.1093:toxsci |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355 |
Biology of Reproduction | Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580 |
Journal of Ovarian Research | DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035 |
Medical Science Monitor | Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845 |
Human & Experimental Toxicology | Palladium nanoparticle effects on endocrine reproductive system of female rats Pubmed:29405765 |
A Pex13 knockout in germ cells induces a spermatogenic arrest volltexte:2018/8921/ | |
Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135 |
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Biological Rhythm Research | Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation … |
Stem Cells Translational Medicine | Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668 |
Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146 |
Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary |
Theriogenology | Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus) |
International Journal of Reproductive Biomedicine | The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study. |
Andrologia | Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Pubmed: 31353623 |
Journal of Ovarian Research | Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718 |
Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938 |
Medicina | Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology Pubmed: 32120970 |
Chemosphere | Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727 |
BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance … Pubmed: 33109277 |
ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043 |
Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic … Pubmed: 32698821 |
Antioxidants (Basel) | Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356 |
chemosphere | N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats Pubmed: 32447106 |
Neurotoxicology | Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice Pubmed: 32407858 |
FASEB JOURNAL | Acyl-CoA synthetase 6 regulates long-chain polyunsaturated fatty acid composition of membrane phospholipids in spermatids and supports normal spermatogenic … Pubmed: 31648559 |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488 |
ACS Applied Bio Materials | Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice |
Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS |
Medical Journal of Cairo University | Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management |
JOURNAL OF ETHNOPHARMACOLOGY | Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655 |
advanced biomedical research | The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome 33457340 |
Endocrinology | Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment 33507237 |
JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368 |
Toxicol Appl Pharmacol | Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion 33965371 |
Nutr Metab | Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells 34039393 |
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researchsquare | Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells |
Scientific reports | Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.) |
Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice | |
JOURNAL OF INHERITED METABOLIC DISEASE | Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in … 34494673 |
Free Radical Biology and Medicine | Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion 34610361 |
Microbiome | Polyamine metabolism links gut microbiota and testicular dysfunction 34758869 |
J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391 |
Cell Death Dis | METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice 34689175 |
Theriogenology | Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with … Pubmed:35063921 |
Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459 |
Sci Total Environ | Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression Pubmed:35276145 |
Ecotoxicology and Environmental Safety | Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero Pubmed:35679725 |
BMC Veterinary Research | A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
APA441Hu62 | Active Luteinizing Hormone (LH) | Cell culture; Activity Assays. |
APA441Hu01 | Active Luteinizing Hormone (LH) | Cell culture; Activity Assays. |
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RPA441Hu02 | Recombinant Luteinizing Hormone (LH) | Positive Control; Immunogen; SDS-PAGE; WB. |
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APA441Hu02 | Active Luteinizing Hormone (LH) | Cell culture; Activity Assays. |
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PAA441Hu08 | Polyclonal Antibody to Luteinizing Hormone (LH) | WB; IHC; ICC; IP. |
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