Active Complement Component 5a (C5a)
- Product No.APA388Mu62
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point9.4
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$
US$
US$
US$
US$
For more details, please contact local distributors!
ACTIVITY TEST
![](/upload/product/pictures/APA388Mu62-202408211525393849.png)
Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 ℃ for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Magazine | Citations |
Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297 |
Journal of Reproductive Immunology | Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845 |
The Journal of Clinical Investigation | Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471 |
Journal of Antimicrobial Chemotherapy | Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587 |
Journal of Orthopaedic Research | Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742 |
SpringerPlus | Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977 |
American Journal fo Reproductive Immunology | Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155 |
Gynecol Obstet Invest | Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247 |
Journal of Neuroimmunology | Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358 |
International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602 |
Digital Repository | Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
Environmental Pollution | Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439 |
Cancer Letters | Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586 |
Acta Paediatrica | Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106 |
Scientific Reports | Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296 |
Nutrients | The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479 |
Journal of NeuroVirology | Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976 |
PLoS One | Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004 |
Frontiers in Immunology | C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855 |
Phytomedicine | Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … |
Aging | C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478 |
STEM CELL REVIEWS AND REPORTS | A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006 |
Modulation of C5a-C5aR interactions against murine mammary cancer cell line | |
FASEB J | C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |
Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
RPA388Mu01 | Recombinant Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA388Mu62 | Eukaryotic Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA388Mu61 | Active Complement Component 5a (C5a) | Cell culture; Activity Assays. |
APA388Mu62 | Active Complement Component 5a (C5a) | Cell culture; Activity Assays. |
EPA388Mu61 | Eukaryotic Complement Component 5a (C5a) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA388Mu01 | Polyclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC; IP. |
LAA388Mu71 | Biotin-Linked Polyclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC. |
MAA388Mu21 | Monoclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC; IP. |
LAA388Mu72 | Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a) | WB; IHC; ICC. |
SEA388Mu | ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA388Mu | Mini Samples ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA388Mu | High Sensitive ELISA Kit for Complement Component 5a (C5a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA388Mu | CLIA Kit for Complement Component 5a (C5a) | Chemiluminescent immunoassay for Antigen Detection. |
LMA388Mu | Multiplex Assay Kit for Complement Component 5a (C5a) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
PSA388Mu01 | Antibody Pair for Complement Component 5a (C5a) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA388Mu01 | ELISA Kit DIY Materials for Complement Component 5a (C5a) | Main materials for "Do It (ELISA Kit) Yourself". |