≡

Active Complement Component 5a (C5a)

ACTIVITY TEST

Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. The activity of recombinant human C5a was also measured by its ability to induce N-acetyl-β-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. 3.2*106 differentiated U937 cells were added to the 24-well plate and different concentrations of rhC5a was added to the 24-well plate and incubated at 37 ℃ for 3min. The cells were centrifuged at 400 g for 3min, and the supernatant contained N-acetyl-β-D-glucosaminidase. The enzyme activity of N-acetyl-β-D-glucosaminidase was measured by the substrate of 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside. The result was shown in figure 1, It was obvious that rhC5a can significantly induce N-acetyl-β-D-glucosaminidase release.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Immunology Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297
Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845
The Journal of Clinical Investigation Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471
Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587
Journal of Orthopaedic Research Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742
SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977
American Journal fo Reproductive Immunology Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155
Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602
Digital Repository Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183
Environmental Pollution Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439
Cancer Letters Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586
Acta Paediatrica Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106
Scientific Reports Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
PLoS One Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004
Frontiers in Immunology C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855
Phytomedicine Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …
Aging C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006
Modulation of C5a-C5aR interactions against murine mammary cancer cell line
FASEB J C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA388Mu01 Recombinant Complement Component 5a (C5a) Positive Control; Immunogen; SDS-PAGE; WB.
EPA388Mu62 Eukaryotic Complement Component 5a (C5a) Positive Control; Immunogen; SDS-PAGE; WB.
APA388Mu61 Active Complement Component 5a (C5a) Cell culture; Activity Assays.
APA388Mu62 Active Complement Component 5a (C5a) Cell culture; Activity Assays.
EPA388Mu61 Eukaryotic Complement Component 5a (C5a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA388Mu01 Polyclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
LAA388Mu71 Biotin-Linked Polyclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC.
MAA388Mu21 Monoclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
LAA388Mu72 Biotin-Linked Monoclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC.
SEA388Mu ELISA Kit for Complement Component 5a (C5a) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA388Mu Mini Samples ELISA Kit for Complement Component 5a (C5a) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA388Mu High Sensitive ELISA Kit for Complement Component 5a (C5a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA388Mu CLIA Kit for Complement Component 5a (C5a) Chemiluminescent immunoassay for Antigen Detection.
LMA388Mu Multiplex Assay Kit for Complement Component 5a (C5a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSA388Mu01 Antibody Pair for Complement Component 5a (C5a) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA388Mu01 ELISA Kit DIY Materials for Complement Component 5a (C5a) Main materials for "Do It (ELISA Kit) Yourself".