Bifunctional ligase/repressor BirA (BirA)
[Edit]Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] ligase; Biotin--protein ligase; Biotin-[acetyl-CoA carboxylase] synthetase
![Bifunctional ligase/repressor BirA (BirA)](/upload/item/img/Bifunctional-ligase-repressor-BirA-(BirA)-X351.png)
The E. coli protein-biotin ligase BirA is extremely specific and covalently attaches biotin to a single lysine in the endogenously expressed BCCP subunit of acetyl-CoA carboxylase2. Additionally, a small synthetic 15-amino-acid peptide is effectively biotinylated by BirA3,4 and fusion of the synthetic biotin acceptor peptide (AP) to a target protein creates an efficient approach for site-specific in vivo and in vitro biotinylation through co-expression or addition of BirA. Although the high specificity and activity of BirA towards the synthetic AP provide a powerful approach for specific labeling, the range of applications for BirA mediated biotinylation is restricted to samples containing AP-fused proteins.
Organism species: Escherichia coli
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Bifunctional ligase/repressor BirA (BirA) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Bifunctional ligase/repressor BirA (BirA) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Bifunctional ligase/repressor BirA (BirA) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Bifunctional ligase/repressor BirA (BirA) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Bifunctional ligase/repressor BirA (BirA) | ELISA Kit Customized Service Offer |