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Non SMC Element 2 Homolog (NSMCE2)

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C8orf36; MMS21; NSE2; Non-structural maintenance of chromosomes element 2 homolog; E3 SUMO-protein ligase NSE2

Non SMC Element 2 Homolog (NSMCE2)
CCC T698
HGNC 26513
MGI 1915751
RGD 1305156
UniProt Q96MF7
The yeast MMS21 protein is a SUMO ligase. hMMS21 stimulates sumoylation of hSMC6 and the DNA repair protein TRAX. Depletion of hMMS21 by RNA interference (RNAi) sensitizes HeLa cells toward DNA damage-induced apoptosis. Ectopic expression of wild-type hMMS21, but not its ligase-inactive mutant, rescues this hypersensitivity of hMMS21-RNAi cells. ATM/ATR are hyperactivated in hMMS21-RNAi cells upon DNA damage. Consistently, hMMS21-RNAi cells show an increased number of phospho-CHK2 foci. hMMS21-RNAi cells show a decreased capacity to repair DNA lesions as measured by the comet assay. SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair in human cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Non SMC Element 2 Homolog (NSMCE2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Non SMC Element 2 Homolog (NSMCE2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Non SMC Element 2 Homolog (NSMCE2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Non SMC Element 2 Homolog (NSMCE2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Non SMC Element 2 Homolog (NSMCE2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Non SMC Element 2 Homolog (NSMCE2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Non SMC Element 2 Homolog (NSMCE2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Non SMC Element 2 Homolog (NSMCE2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Non SMC Element 2 Homolog (NSMCE2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Non SMC Element 2 Homolog (NSMCE2) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Human MMS21/NSE2 is a SUMO ligase required for DNA repair."Mol. Cell. Biol. 25:7021-7032(2005) [PubMed] [Europe PMC] [Abstract]
  4. "Human SMC5/6 complex promotes sister chromatid homologous recombination by recruiting the SMC1/3 cohesin complex to double-strand breaks."EMBO J. 25:3377-3388(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins."Nat. Struct. Mol. Biol. 14:581-590(2007) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex."Mol. Cell. Biol. 28:1197-1206(2008) [PubMed] [Europe PMC] [Abstract]
  7. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  8. "SMC5 and MMS21 are required for chromosome cohesion and mitotic progression."Cell Cycle 8:2211-2218(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Solution structure of the SP-RING domain in non-SMC element 2 homolog (MMS21, S. cerevisiae)."Submitted (OCT-2007) to the PDB data bank
  13. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]