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Cullin 8 (CUL8)

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RTT101; Cullin-C; Regulator of Ty1 transposition protein 101

Cullin 8 (CUL8)
CCC S841
PubMed 12676951
UniProt P47050
Cul3 and are functionally distinct from Cdc53 and do not interact with ySkp1, suggesting that they target substrates by Skp1- and possibly F-box protein-independent mechanisms. Whereas null mutants of CUL3 appear normal, yeast cells lacking CUL8 have a slower growth rate and are delayed in their progress through anaphase.
The anaphase delay phenotype can be complemented by ectopic expression of Cul8 but not by any other yeast or human cullins, nor by a cul8 mutant deficient in binding to RING finger protein Roc1. Deletion of the RAD9 gene suppressed the anaphase delay phenotype of cul8, suggesting that loss of Cul8 function may compromise genomic integrity. These results indicate that in addition to the anaphase promoting complex, mitotic progression may involve another E3 ubiquitin ligase mediated by Cul8 protein.

Organism species: S. cerevisiae (Yeast)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 8 (CUL8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 8 (CUL8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 8 (CUL8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 8 (CUL8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 8 (CUL8) ELISA Kit Customized Service Offer
  1. "A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression."J. Biol. Chem. 278:22828-22837(2003) [PubMed] [Europe PMC] [Abstract]
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
  3. "The reference genome sequence of Saccharomyces cerevisiae: Then and now."G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
  4. "Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance."Genetics 159:1449-1465(2001) [PubMed] [Europe PMC] [Abstract]
  5. "Global analysis of protein localization in budding yeast."Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Saccharomyces cerevisiae ubiquitin-like protein Rub1 conjugates to cullin proteins Rtt101 and Cul3 in vivo."Biochem. J. 377:459-467(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The cullin Rtt101p promotes replication fork progression through damaged DNA and natural pause sites."Curr. Biol. 16:786-792(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Budding yeast Mms22 and Mms1 regulate homologous recombination induced by replisome blockage."DNA Repair 7:811-818(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Rtt101 and Mms1 in budding yeast form a CUL4(DDB1)-like ubiquitin ligase that promotes replication through damaged DNA."EMBO Rep. 9:1034-1040(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Regulation of rtt107 recruitment to stalled DNA replication forks by the cullin rtt101 and the rtt109 acetyltransferase."Mol. Biol. Cell 19:171-180(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A role for ubiquitin in the clearance of nonfunctional rRNAs."Genes Dev. 23:963-974(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Cul8/Rtt101 forms a variety of protein complexes that regulate DNA damage response and transcriptional silencing."J. Biol. Chem. 285:9858-9867(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Ubiquitylation of FACT by the cullin-E3 ligase Rtt101 connects FACT to DNA replication."Genes Dev. 24:1485-1490(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Mms1 and Mms22 stabilize the replisome during replication stress."Mol. Biol. Cell 22:2396-2408(2011) [PubMed] [Europe PMC] [Abstract]
  15. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  16. "A Cul4 E3 ubiquitin ligase regulates histone hand-off during nucleosome assembly."Cell 155:817-829(2013) [PubMed] [Europe PMC] [Abstract]