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Mediator Complex Subunit 5 (MED5)

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NUT1; Negative Regulator Of URS2 Protein 1; Mediator of RNA polymerase II transcription subunit 5

Mediator Complex Subunit 5 (MED5)
CCC S707
PubMed 16230344
UniProt P53114
MED5, belongs to the Mediator complex subunit 5 family, Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II.

Organism species: S. cerevisiae (Yeast)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 5 (MED5) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 5 (MED5) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 5 (MED5) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 5 (MED5) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 5 (MED5) ELISA Kit Customized Service Offer
  1. "DNA sequence analysis of a 35 kb segment from Saccharomyces cerevisiae chromosome VII reveals 19 open reading frames including RAD54, ACE1/CUP2, PMR1, RCK1, AMS1 and CAL1/CDC43."Yeast 11:1413-1419(1995) [PubMed] [Europe PMC] [Abstract]
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
  3. "The reference genome sequence of Saccharomyces cerevisiae: Then and now."G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
  4. "Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae."J. Biol. Chem. 273:30851-30854(1998) [PubMed] [Europe PMC] [Abstract]
  5. "Nuclear proteins Nut1p and Nut2p cooperate to negatively regulate a Swi4p-dependent lacZ reporter gene in Saccharomyces cerevisiae."Mol. Cell. Biol. 18:4707-4718(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Global analysis of protein localization in budding yeast."Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Global analysis of protein expression in yeast."Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Association of the Mediator complex with enhancers of active genes."Proc. Natl. Acad. Sci. U.S.A. 100:13887-13891(2003) [PubMed] [Europe PMC] [Abstract]
  9. "A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II." Mol. Cell 14:553-557(2004) [PubMed] [Europe PMC] [Abstract]
  10. "A high resolution protein interaction map of the yeast Mediator complex."Nucleic Acids Res. 32:5379-5391(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Preponderance of free mediator in the yeast Saccharomyces cerevisiae."J. Biol. Chem. 280:31200-31207(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription in yeast extracts."J. Biol. Chem. 280:33739-33748(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Mediator expression profiling epistasis reveals a signal transduction pathway with antagonistic submodules and highly specific downstream targets."Mol. Cell 19:511-522(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Mediator as a general transcription factor."J. Biol. Chem. 281:80-89(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Activator-specific recruitment of Mediator in vivo."Nat. Struct. Mol. Biol. 13:117-120(2006) [PubMed] [Europe PMC] [Abstract]
  16. "Med19(Rox3) regulates intermodule interactions in the Saccharomyces cerevisiae mediator complex."J. Biol. Chem. 282:5551-5559(2007) [PubMed] [Europe PMC] [Abstract]
  17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Structure of the yeast RNA polymerase II holoenzyme: mediator conformation and polymerase interaction."Mol. Cell 10:409-415(2002) [PubMed] [Europe PMC] [Abstract]