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Phospholipase B Domain Containing Protein 2 (PLBD2)

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p76; PLB Homolog 2; Mannose-6-Phosphate Protein Associated Protein p76; LAMA-like protein 2; Lamina ancestor homolog 2; Phospholipase B domain-containing protein 2

Phospholipase B Domain Containing Protein 2 (PLBD2)
CCC R726
HGNC 27283
MGI 1919022
PubMed 11577733
RGD 708461
UniProt Q8NHP8
Most soluble lysosomal proteins carry Man6P (mannose 6-phosphate), a specific carbohydrate marker that enables their binding to cellular MPRs (Man6P receptors) and their subsequent targeting towards the lysosome.
This characteristic was exploited to identify novel soluble lysosomal proteins by proteomic analysis of Man6P proteins purified from a human cell line. The presence of Man6P sugars was confirmed by an MPR overlay experiment, which showed the direct and Man6P-dependent interaction between p76 and the MPR. The presence of six N-glycosylation sites was validated by progressive peptide-N-glycosidase F deglycosylation. Experiments using N- and C-termini directed anti-p76 antibodies provided insights into p76 maturation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phospholipase B Domain Containing Protein 2 (PLBD2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phospholipase B Domain Containing Protein 2 (PLBD2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phospholipase B Domain Containing Protein 2 (PLBD2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phospholipase B Domain Containing Protein 2 (PLBD2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phospholipase B Domain Containing Protein 2 (PLBD2) ELISA Kit Customized Service Offer
  1. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Biochemical characterization and lysosomal localization of the mannose-6-phosphate protein p76 (hypothetical protein LOC196463)."Biochem. J. 402:449-458(2007) [PubMed] [Europe PMC] [Abstract]
  4. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
  5. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]