≡

Proteasomal ATPase Associated Factor 1 (PAAF1)

[Edit]

WDR71; Rpn14; WD Repeat Domain 71

Proteasomal ATPase Associated Factor 1 (PAAF1)
CCC R464
HGNC 25687
PubMed 22316138
UniProt Q9BRP4
Wiki PAAF1
Immunoaffinity purification of PAAF1 confirmed its interaction with the 19S regulatory complex and further showed that the 19S regulatory complex bound with PAAF1 was not stably associated with the 20S core. Overexpression of PAAF1 in HeLa cells decreased the level of the 20S core associated with the 19S complex in a dose-dependent fashion, suggesting that PAAF1 binding to proteasomal ATPases inhibited the assembly of the 26S proteasome. Proteasomal degradation assays using reporters based on green fluorescent protein revealed that overexpression of PAAF1 inhibited the proteasome activity in vivo. Furthermore, the suppression of PAAF1 expression that is mediated by small inhibitory RNA enhanced the proteasome activity. These results suggest that PAAF1 functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Proteasomal ATPase Associated Factor 1 (PAAF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Proteasomal ATPase Associated Factor 1 (PAAF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Proteasomal ATPase Associated Factor 1 (PAAF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Proteasomal ATPase Associated Factor 1 (PAAF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Proteasomal ATPase Associated Factor 1 (PAAF1) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases."Mol. Cell. Biol. 25:3842-3853(2005) [PubMed] [Europe PMC] [Abstract]
  5. "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex."Biochemistry 46:3553-3565(2007) [PubMed] [Europe PMC] [Abstract]
  6. "The proteasome regulates HIV-1 transcription by both proteolytic and nonproteolytic mechanisms."Mol. Cell 25:369-383(2007) [PubMed] [Europe PMC] [Abstract]
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  8. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  9. "Spt6 levels are modulated by PAAF1 and proteasome to regulate the HIV-1 LTR."Retrovirology 9:13-13(2012) [PubMed] [Europe PMC] [Abstract]