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Vps9 Domain And Ankyrin Repeat Containing Protein (VARP)

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ANKRD27; Ankyrin Repeat Domain Protein 27

Vps9 Domain And Ankyrin Repeat Containing Protein (VARP)
CCC R389
HGNC 25310
MGI 2444103
PubMed 19403694
RGD 1307106
UniProt Q96NW4
The small GTPases Rab5 and Rab21 are closely related, and play essential roles in endocytic trafficking. Rab5 is regulated by VPS9-domain-containing guanine nucleotide exchange factors. Varp interacts preferentially with GDP-bound Rab21 and has a much stronger guanine nucleotide exchange activity towards Rab21 than Rab5. Furthermore, RNAi-mediated depletion of endogenous Varp significantly disrupts the activity of Rab21 in HeLa cells. Ectopically expressed Varp mainly localizes to early endosomes and causes enlargement of early endosomes and giant late endosomes. Both the VPS9 domain and ankyrin-repeats are required for the endosomal localization and the activity of Varp in vivo. These results suggest that Varp is a potential Rab21 guanine nucleotide exchange factor and might regulate endosome dynamics in vivo.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) CLIA Kit Customized Service Offer
n/a ELISA Kit for Vps9 Domain And Ankyrin Repeat Containing Protein (VARP) ELISA Kit Customized Service Offer
  1. "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Large-scale cDNA transfection screening for genes related to cancer development and progression." Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics."J. Cell Sci. 119:1053-1062(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Varp interacts with Rab38 and functions as its potential effector."Biochem. Biophys. Res. Commun. 372:162-167(2008) [PubMed] [Europe PMC] [Abstract]
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth."EMBO Rep. 10:1117-1124(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "A molecular network for the transport of the TI-VAMP/VAMP7 vesicles from cell center to periphery."Dev. Cell 23:166-180(2012) [PubMed] [Europe PMC] [Abstract]
  12. "The binding of Varp to VAMP7 traps VAMP7 in a closed, fusogenically inactive conformation."Nat. Struct. Mol. Biol. 19:1300-1309(2012) [PubMed] [Europe PMC] [Abstract]
  13. "VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface."Dev. Cell 29:591-606(2014) [PubMed] [Europe PMC] [Abstract]