Zbed3 contains a PPPPSPT motif, which is crucial to its binding to Axin. The Ser and Thr residues in the motif appear to be also phosphorylated by glycogen synthase kinase 3β (GSK3β) and the CKI family kinases, as GSK3β and CKIϵ could enhance the interaction of Zbed3 with Axin. Expressing Zbed3, but not these mutants, led to inhibition of GSK3β-mediated β-catenin phosphorylation, cytoplasmic β-catenin accumulation, and activation of lymphoid enhancer binding factor-1-dependent reporter gene transcription. Furthermore, knockdown of Zbed3 with RNA interference attenuated Wnt-induced β-catenin accumulation, lymphoid enhancer binding factor-1-dependent luciferase reporter activity, and the Wnt target gene expression. Zbed3 is a novel Axin-binding protein that is involved in Wnt/β-catenin signaling modulation.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPQ870Hu01 | Recombinant Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | MAQ870Hu21 | Monoclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | WB; IHC; ICC; IP. |
PAQ870Hu01 | Polyclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | WB; IHC; ICC; IP. | |
Assay Kits | SEQ870Hu | ELISA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCQ870Hu | CLIA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Chemiluminescent immunoassay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Zinc Finger, BED Type Containing Protein 3 (ZBED3) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Zinc Finger, BED Type Containing Protein 3 (ZBED3) | ELISA Kit Customized Service Offer |
- "The DNA sequence and comparative analysis of human chromosome 5." Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]