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Fem-1 Homolog B (FEM1B)

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F1AA; Fem-1-like death receptor-binding protein alpha; Fem-1-like in apoptotic pathway protein alpha

Fem-1 Homolog B (FEM1B)
CCC Q060
HGNC 3649
MGI 1335087
PubMed 19908242
RGD 1304569
UniProt Q9UK73
Depletion of FEM1B by small interfering RNA in cancer cells impairs the activation of CHK1 kinase activity and attenuates the induction of CHK1 Ser345 phosphorylation upon replication interference. It is to be noted that, CHK2 Thr68 phosphorylation is not altered by FEM1B downregulation.
FEM1B is able to associate with chromatin, and such association facilitates chromatin loading of the Rad9 protein. Consistently, ATR activity is poorly maintained in FEM1B knockdown cells; and FEM1B-ablated cells are as sensitive to replication block as CHK1-depleted cells. FEM1B, which acts as a bridge linking CHK1 and Rad9, thus facilitating checkpoint signaling induced by replication stress.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Fem-1 Homolog B (FEM1B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Fem-1 Homolog B (FEM1B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Fem-1 Homolog B (FEM1B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Fem-1 Homolog B (FEM1B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Fem-1 Homolog B (FEM1B) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Fem-1 Homolog B (FEM1B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Fem-1 Homolog B (FEM1B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Fem-1 Homolog B (FEM1B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Fem-1 Homolog B (FEM1B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Fem-1 Homolog B (FEM1B) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Fem-1 Homolog B (FEM1B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Fem-1 Homolog B (FEM1B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Fem-1 Homolog B (FEM1B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Fem-1 Homolog B (FEM1B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Fem-1 Homolog B (FEM1B) ELISA Kit Customized Service Offer
  1. "F1Aalpha, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis."J. Biol. Chem. 274:32461-32468(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Sequence, organization, and expression of the human FEM1B gene."Biochem. Biophys. Res. Commun. 267:317-320(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 4:307-313(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Analysis of the DNA sequence and duplication history of human chromosome 15." Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "The Caenorhabditis elegans sex-determining protein fem-2 and its human homologue, hFEM-2, are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis."J. Biol. Chem. 276:44193-44202(2001) [PubMed] [Europe PMC] [Abstract]
  9. "VHL-box and SOCS-box domains determine binding specificity for Cul2-Rbx1 and Cul5-Rbx2 modules of ubiquitin ligases."Genes Dev. 18:3055-3065(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Human FEM1B is required for Rad9 recruitment and CHK1 activation in response to replication stress."Oncogene 28:1971-1981(2009) [PubMed] [Europe PMC] [Abstract]