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Supernatant Protein Factor (SPF)

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SEC14L2; TAP; C22orf6; SEC14-like protein 2; Alpha-tocopherol-associated protein; Squalene transfer protein

Supernatant Protein Factor (SPF)
CCC P703
HGNC 10699
MGI 1915065
PubMed 11226224
RGD 621779
UniProt O76054
Wiki SPF
SEC14L2 stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. The deduced 403-amino acid protein has a calculated molecular mass of about 46 kD and contains a conserved hydrophobic lipid-binding pocket called the CRAL (cis-retinal binding)/TRIO (triple function) domain.
Human and bovine TAP share about 84% amino acid identity. mRNA dot blot analysis detected broad tissue distribution, with highest expression in liver, prostate, and brain. Northern blot analysis detected a major transcript of 2.8 kb and 2 minor transcripts of approximately 4.2 and 2.8 kb expressed at highest levels in liver and brain and more weakly in kidney.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Supernatant Protein Factor (SPF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Supernatant Protein Factor (SPF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Supernatant Protein Factor (SPF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Supernatant Protein Factor (SPF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Supernatant Protein Factor (SPF) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Supernatant Protein Factor (SPF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Supernatant Protein Factor (SPF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Supernatant Protein Factor (SPF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Supernatant Protein Factor (SPF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Supernatant Protein Factor (SPF) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Supernatant Protein Factor (SPF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Supernatant Protein Factor (SPF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Supernatant Protein Factor (SPF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Supernatant Protein Factor (SPF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Supernatant Protein Factor (SPF) ELISA Kit Customized Service Offer
  1. "A novel human tocopherol-associated protein: cloning, in vitro expression, and characterization."J. Biol. Chem. 275:25672-25680(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Tocopherol-associated protein is a ligand-dependent transcriptional activator."Biochem. Biophys. Res. Commun. 285:295-299(2001) [PubMed] [Europe PMC] [Abstract]
  3. "Supernatant protein factor, which stimulates the conversion of squalene to lanosterol, is a cytosolic squalene transfer protein and enhances cholesterol biosynthesis."Proc. Natl. Acad. Sci. U.S.A. 98:2244-2249(2001) [PubMed] [Europe PMC] [Abstract]
  4. "Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain."DNA Res. 6:329-336(1999) [PubMed] [Europe PMC] [Abstract]
  5. "A genome annotation-driven approach to cloning the human ORFeome."Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "The DNA sequence of human chromosome 22." Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  10. "Crystal structure of the human supernatant protein factor."Structure 10:1533-1540(2002) [PubMed] [Europe PMC] [Abstract]