Zinc Finger Protein 335 (ZNF335)

ZNF335 enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure.

The deduced 1,342-amino acid NIF1 protein contains 6 predicted C2H2 zinc fingers, of which the first 3 comprise a BED finger DNA-binding domain; an LXXLL motif; a C-terminal leucine zipper region; an N-terminal 35-amino acid region enriched in acidic residues; and putative protein kinase A and tyrosine kinase phosphorylation sites. NIF1 acts as a regulator or cotransducer of NCOA6 coactivator function.