Zinc Finger Protein 296 (ZNF296)

The deduced 347-amino acid human protein shares 67.3% homology with the 333-amino acid mouse protein. Both contain 6 C2H2-type zinc finger motifs, 6 putative phosphorylation sites, and 7 N-myristoylation sites. RT-PCR analysis of mouse tissues detected highest Znf296 expression in testis and embryonic stem cells. Znf296 was also expressed in bone marrow. In situ hybridization of mouse testis of various developmental stages revealed Znf296 expression in condensing spermatids of adult mouse testis only.The translocation resulted in a derivative chromosome 17 containing the promoter/enhancer region of the MPO gene fused to the promoter and coding region of the ZNF296 gene, resulting in 90-fold upregulation of ZNF296 expression in the patient's leukemia cells.