Dickkopf Like Protein 1 (DKKL1)

The deduced 242-amino acid SGY protein, which is 22% identical to DKK3 and 59% identical to the mouse Sgy sequence, possesses 2 potential N-glycosylation sites. Northern blot and in situ hybridization analysis revealed expression of a 1.0-kb Sgy transcript in adult mouse testis and day-11 and -15 embryos. Western blot analysis showed that SGY is secreted as a 40- to 50-kD protein; deglycosylation reduced the protein by 5 to 10 kD. Functional analysis determined that SGY does not block Xenopus Wnt8, Wnt3a, or Wnt2b induction of a secondary axis in frog embryos.

Scott (2000) mapped the SGY gene to chromosome 19 based on sequence similarity between the SGY sequence and the chromosome 19 clones LLNLR-254A7 and LLNLR-278H5 .