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Autophagy Related Protein 3 (ATG3)

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APG3L; APG3 Autophagy 3-Like; Ubiquitin-like-conjugating enzyme ATG3

Autophagy Related Protein 3 (ATG3)
CCC N502
HGNC 20962
MGI 1915091
PubMed 20723759
RGD 708464
UniProt Q9NT62
ATG3 formed an enzyme-substrate intermediate via a thiol ester bond that showed rapid turnover. Replacement of the active-site cysteine of ATG3 with serine (C264S) resulted in the formation of a stable O-ester bond that allowed visualization of stable intermediates. Overexpression of ATG7 enhanced the formation of stable E2-substrate complexes between ATG3-C264S and each of the Apg8 homologs. MAPLC3 was the preferred substrate, and the reaction with MAPLC3 occurred predominantly in the cytosol. Coimmunoprecipitation of ATG7 with ATG3 indicated that ATG3 forms an E1-E2 complex with ATG7, similar to the yeast Apg3-Apg7 complex. Overexpression of ATG3 facilitated formation of the ATG12-ATG5 conjugate, suggesting that ATG3 cross-talks with the ATG12 conjugation system.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Autophagy Related Protein 3 (ATG3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Autophagy Related Protein 3 (ATG3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Autophagy Related Protein 3 (ATG3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Autophagy Related Protein 3 (ATG3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Autophagy Related Protein 3 (ATG3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Autophagy Related Protein 3 (ATG3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Autophagy Related Protein 3 (ATG3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Autophagy Related Protein 3 (ATG3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Autophagy Related Protein 3 (ATG3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Autophagy Related Protein 3 (ATG3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Autophagy Related Protein 3 (ATG3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Autophagy Related Protein 3 (ATG3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Autophagy Related Protein 3 (ATG3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Autophagy Related Protein 3 (ATG3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Autophagy Related Protein 3 (ATG3) ELISA Kit Customized Service Offer
  1. "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p."J. Biol. Chem. 277:13739-13744(2002) [PubMed] [Europe PMC] [Abstract]
  2. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing."Biochem. Biophys. Res. Commun. 296:1164-1170(2002) [PubMed] [Europe PMC] [Abstract]
  6. "The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification."J. Biol. Chem. 278:39517-39526(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  9. "Atg8L/Apg8L is the fourth mammalian modifier of mammalian Atg8 conjugation mediated by human Atg4B, Atg7 and Atg3."FEBS J. 273:2553-2562(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  11. "A novel hybrid yeast-human network analysis reveals an essential role for FNBP1L in antibacterial autophagy."J. Immunol. 182:4917-4930(2009) [PubMed] [Europe PMC] [Abstract]
  12. "ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death."Cell 142:590-600(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Cleavage of Atg3 protein by caspase-8 regulates autophagy during receptor-activated cell death."Apoptosis 17:810-820(2012) [PubMed] [Europe PMC] [Abstract]
  15. "The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation."Autophagy 8:88-97(2012) [PubMed] [Europe PMC] [Abstract]
  16. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]