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Smg-9 Homolog (SMG9)

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C19orf61

Smg-9 Homolog (SMG9)
CCC N327
HGNC 25763
MGI 1919247
RGD 1309888
UniProt Q9H0W8
Using reciprocal immunoprecipitation analysis, Yamashita et al. (2009) showed that SMG8 and SMG9 tightly associated with SMG1 in HeLa cell lysates, and that SMG9 appeared to mediate the interaction. Both SMG8 and SMG9 suppressed the kinase activity of SMG1, and both were SMG1 target substrates. Depletion of SMG9 resulted in accumulation of phosphorylated UPF1. SMG8 was required to recruit SMG1 to the surveillance complex, and depletion of SMG8 caused accumulation of a complex containing the ribosome, UPF1, ERF1, ERF3, and the EJC on the spliced mRNA-protein complex. The deduced 520-amino acid protein has a central NTPase domain and is highly conserved in many metazoans, but not in yeast or Arabidopsis thaliana. SMG9 had an apparent molecular mass of 60 kD by SDS-PAGE.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Smg-9 Homolog (SMG9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Smg-9 Homolog (SMG9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Smg-9 Homolog (SMG9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Smg-9 Homolog (SMG9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Smg-9 Homolog (SMG9) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Smg-9 Homolog (SMG9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Smg-9 Homolog (SMG9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Smg-9 Homolog (SMG9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Smg-9 Homolog (SMG9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Smg-9 Homolog (SMG9) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Smg-9 Homolog (SMG9) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Smg-9 Homolog (SMG9) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Smg-9 Homolog (SMG9) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Smg-9 Homolog (SMG9) CLIA Kit Customized Service Offer
n/a ELISA Kit for Smg-9 Homolog (SMG9) ELISA Kit Customized Service Offer
  1. "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  6. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  8. "SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay."Genes Dev. 23:1091-1105(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8."Genes Dev. 25:153-164(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Characterization of SMG-9, an essential component of the nonsense-mediated mRNA decay SMG1C complex."Nucleic Acids Res. 39:347-358(2011) [PubMed] [Europe PMC] [Abstract]
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]