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Phosphoseryl tRNA Kinase (PSTK)

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C10orf89; O-phosphoseryl-tRNA(Sec) kinase; L-seryl-tRNA(Sec) kinase

Phosphoseryl tRNA Kinase (PSTK)
CCC M952
HGNC 28578
MGI 2685945
UniProt Q8IV42
In eukaryotes, decoding of the UGA codon as selenocysteine (Sec) requires a Sec insertion sequence (SECIS) in the 3-prime untranslated region of the mRNA. A specific elongation factor, EFSEC, specifically recognizes selenocysteyl-tRNA(Ser)Sec and the SECIS and directs insertion of Sec into protein.The PSTK cDNA was isolated and cloned into a His-tag expression vector. The deduced human PSTK protein contains 359 amino acids with a predicted ATP-binding domain.
PSTK specifically catalyzed the formation of phosphoseryl-tRNA(Ser)Sec from seryl-tRNA(Ser)Sec and ATP in the presence of Mg2+. In binding assays, both tRNA(Ser)Sec and seryl-tRNA(Ser)Sec bound to PSTK, but ATP enhanced the binding of tRNA(Ser)Sec and decreased the binding of seryl-tRNA(Ser)Sec.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphoseryl tRNA Kinase (PSTK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphoseryl tRNA Kinase (PSTK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphoseryl tRNA Kinase (PSTK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphoseryl tRNA Kinase (PSTK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphoseryl tRNA Kinase (PSTK) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphoseryl tRNA Kinase (PSTK) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphoseryl tRNA Kinase (PSTK) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphoseryl tRNA Kinase (PSTK) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphoseryl tRNA Kinase (PSTK) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphoseryl tRNA Kinase (PSTK) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]