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Aprataxin And PNKP Like Factor (APLF)

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C2orf13; PALF; XIP1; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1

Aprataxin And PNKP Like Factor (APLF)
CCC M938
HGNC 28724
MGI 1919353
PubMed 23178593
RGD 1565557
UniProt Q8IW19
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks. APLF interacts with both XRCC1 and XRCC4, the same components of the single- and double-strand break repair machinery that are bound by PNK and APTX, but not with APTX or DNA ligase III (LIG3).
The FHA domain of APLF interacts with XRCC1, and that both proteins colocalize in the cell at sites of laser-induced UVA damage. The interaction of XRCC1 with APLF was largely dependent on the phosphorylation of XRCC1 by casein kinase-2 (CK2). YFP-tagged APLF accumulated in nuclear foci in the absence of XRCC1 but this was disrupted by mutations in the zinc finger-like motifs.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aprataxin And PNKP Like Factor (APLF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aprataxin And PNKP Like Factor (APLF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aprataxin And PNKP Like Factor (APLF) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aprataxin And PNKP Like Factor (APLF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aprataxin And PNKP Like Factor (APLF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aprataxin And PNKP Like Factor (APLF) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Aprataxin And PNKP Like Factor (APLF) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Aprataxin And PNKP Like Factor (APLF) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Aprataxin And PNKP Like Factor (APLF) CLIA Kit Customized Service Offer
n/a ELISA Kit for Aprataxin And PNKP Like Factor (APLF) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "A novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses."EMBO J. 26:2094-2103(2007) [PubMed] [Europe PMC] [Abstract]
  5. "Human Xip1 (C2orf13) is a novel regulator of cellular responses to DNA strand breaks."J. Biol. Chem. 282:19638-19643(2007) [PubMed] [Europe PMC] [Abstract]
  6. "APLF (C2orf13) is a novel human protein involved in the cellular response to chromosomal DNA strand breaks."Mol. Cell. Biol. 27:3793-3803(2007) [PubMed] [Europe PMC] [Abstract]
  7. "APLF (C2orf13) facilitates nonhomologous end-joining and undergoes ATM-dependent hyperphosphorylation following ionizing radiation."DNA Repair 7:292-302(2008) [PubMed] [Europe PMC] [Abstract]
  8. "APLF (C2orf13) is a novel component of poly(ADP-ribose) signaling in mammalian cells."Mol. Cell. Biol. 28:4620-4628(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins."Nature 451:81-85(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose)."Nat. Struct. Mol. Biol. 17:241-243(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Structure and identification of ADP-ribose recognition motifs of APLF and role in the DNA damage response."Proc. Natl. Acad. Sci. U.S.A. 107:9129-9134(2010) [PubMed] [Europe PMC] [Abstract]