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SET Domain Containing Protein 8 (SETD8)

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SET8; SET07; PR-Set7; KMT5A; Histone-lysine N-methyltransferase SETD8; H4-K20-HMTase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07

SET Domain Containing Protein 8 (SETD8)
CCC M839
HGNC 29489
MGI 1915206
PubMed 22117221
RGD 1595707
UniProt Q9NQR1
Wiki SETD8
A mutation in Drosophila Pr/set7 was lethal: second instar larval death coincided with the loss of H4-lys20 methylation, indicating a fundamental role for Pr/set7 in development. Transcriptionally competent regions lacked H4-lys20 methylation, but the modification coincided with condensed chromosomal regions on polytene chromosomes, including chromocenter and euchromatic arms. The Drosophila male X chromosome, which is hyperacetylated at H4-lys16, had significantly decreased levels of lys20 methylation compared with that of females. In vitro, methylation of lys20 and acetylation of lys16 on the H4 tail were competitive. Taken together, these results supported the hypothesis that methylation of H4-lys20 maintains silent chromatin, in part, by precluding neighboring acetylation on the H4 tail.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SET Domain Containing Protein 8 (SETD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SET Domain Containing Protein 8 (SETD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SET Domain Containing Protein 8 (SETD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SET Domain Containing Protein 8 (SETD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for SET Domain Containing Protein 8 (SETD8) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SET Domain Containing Protein 8 (SETD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SET Domain Containing Protein 8 (SETD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SET Domain Containing Protein 8 (SETD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SET Domain Containing Protein 8 (SETD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for SET Domain Containing Protein 8 (SETD8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SET Domain Containing Protein 8 (SETD8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SET Domain Containing Protein 8 (SETD8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SET Domain Containing Protein 8 (SETD8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SET Domain Containing Protein 8 (SETD8) CLIA Kit Customized Service Offer
n/a ELISA Kit for SET Domain Containing Protein 8 (SETD8) ELISA Kit Customized Service Offer
  1. "PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin."Mol. Cell 9:1201-1213(2002) [PubMed] [Europe PMC] [Abstract]
  2. "Purification and functional characterization of SET8, a nucleosomal histone H4-lysine 20-specific methyltransferase."Curr. Biol. 12:1086-1099(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes."Genes Dev. 16:2225-2230(2002) [PubMed] [Europe PMC] [Abstract]
  6. "A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1."Mol. Cell 14:713-725(2004) [PubMed] [Europe PMC] [Abstract]
  7. "SET8 recognizes the sequence RHRK20VLRDN within the N terminus of histone H4 and mono-methylates lysine 20."J. Biol. Chem. 280:30025-30031(2005) [PubMed] [Europe PMC] [Abstract]
  8. "A trans-tail histone code defined by monomethylated H4 Lys-20 and H3 Lys-9 demarcates distinct regions of silent chromatin."J. Biol. Chem. 281:12760-12766(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Modulation of p53 function by SET8-mediated methylation at lysine 382."Mol. Cell 27:636-646(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Histone H4 lysine 20 monomethylation promotes transcriptional repression by L3MBTL1."Oncogene 27:4293-4304(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "The MBT repeats of L3MBTL1 link SET8-mediated p53 methylation at lysine 382 to target gene repression."J. Biol. Chem. 285:37725-37732(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  14. "The tumor suppressor SirT2 regulates cell cycle progression and genome stability by modulating the mitotic deposition of H4K20 methylation."Genes Dev. 27:639-653(2013) [PubMed] [Europe PMC] [Abstract]
  15. "CRL1-FBXO11 promotes Cdt2 ubiquitylation and degradation and regulates Pr-Set7/Set8-mediated cellular migration."Mol. Cell 49:1147-1158(2013) [PubMed] [Europe PMC] [Abstract]
  16. "Specificity and mechanism of the histone methyltransferase Pr-Set7."Genes Dev. 19:1444-1454(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Structural and functional analysis of SET8, a histone H4 'Lys-20' methyltransferase."Genes Dev. 19:1455-1465(2005) [PubMed] [Europe PMC] [Abstract]