≡

SEC16 Homolog A (SEC16A)

[Edit]

p250; Protein transport protein Sec16A

SEC16 Homolog A (SEC16A)
CCC M773
HGNC 29006
MGI 2139207
PubMed 22347445
RGD 620478
UniProt O15027
Wiki SEC16A
SEC16A is a peripheral membrane protein that defines ERES in mammalian cells and is required for protein transport from ER to Golgi.The predicted 2,357 amino acid protein contains 3 proline-rich regions and 1 tyrosine-rich region. SEC16A shares 19.8% amino acid identity with S. cerevisiae Sec16a. By GST pull-down assay, the authors identified mouse Sec16a, which they called p250, as a Sec23a -interacting protein. Immunofluorescence microscopy localized SEC16A to ERES, where it colocalized with COPII component SEC31A and with ER-Golgi intermediate compartment marker ERGIC53 (LMAN1).SAR1 is required for SEC16A association with ERES and formation of the ERES scaffold and that there is a stoichiometric level of SEC16A required for optimal ER-to-Golgi protein transport.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SEC16 Homolog A (SEC16A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SEC16 Homolog A (SEC16A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SEC16 Homolog A (SEC16A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SEC16 Homolog A (SEC16A) CLIA Kit Customized Service Offer
n/a ELISA Kit for SEC16 Homolog A (SEC16A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SEC16 Homolog A (SEC16A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SEC16 Homolog A (SEC16A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SEC16 Homolog A (SEC16A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SEC16 Homolog A (SEC16A) CLIA Kit Customized Service Offer
n/a ELISA Kit for SEC16 Homolog A (SEC16A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SEC16 Homolog A (SEC16A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SEC16 Homolog A (SEC16A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SEC16 Homolog A (SEC16A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SEC16 Homolog A (SEC16A) CLIA Kit Customized Service Offer
n/a ELISA Kit for SEC16 Homolog A (SEC16A) ELISA Kit Customized Service Offer
  1. "Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization."Mol. Biol. Cell 18:839-849(2007) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 9." Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Sec16 defines endoplasmic reticulum exit sites and is required for secretory cargo export in mammalian cells."Traffic 7:1678-1687(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  11. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  18. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]