Zinc Finger, HIT-Type Protein 2 (ZNHIT2)

FON, which contains only 1 exon, is positioned in the same transcriptional orientation as FAU, which is 4 kb upstream. C11ORF5 is located 171 nucleotides upstream of TM7SF2 in a tail-to-tail configuration. The C11ORF5 cDNA encodes a deduced 403-amino acid acidic (pI 5.8) protein predicted to have 11 phosphorylation and 6 N-myristoylation sites. The human and mouse C11ORF5 proteins have 81% sequence identity. Northern blot analysis revealed low expression of a 1.4-kb transcript in all tissues tested except for testis in which high expression was seen. Additional 4- and 7-kb transcripts were seen in skeletal muscle and heart. In mice, in situ hybridization revealed high expression of C11orf5 in adult testis, particularly in seminiferous tubules, and lower expression in all other tissues tested.