V-Myc Myelocytomatosis Viral Oncogene Homolog 2 (MYCL2)

By somatic cell hybridization and in situ hybridization, they assigned the gene to 1p32. This LMYC sequence was amplified 10- to 20-fold in the DNA of 4 SCCL lines and of 1 SCCL specimen taken directly from a patient. A restriction polymorphism was found. In heterozygotes, only 1 of the 2 alleles was amplified in any 1 genome.

Morton et al. (1989) characterized a DNA sequence with homology to the MYC family of protooncogenes and found that it is a processed gene related to L-MYC (MYCL1). This processed gene, designated MYCL2, was isolated by cross hybridization to an oligonucleotide probe synthesized from the MYC sequence in a highly conserved region. MYCL2 showed an open reading frame of 1,194 basepairs with no intervening sequences and strong homology to the sequence of MYCL1. Mapping of MYCL2 by somatic cell hybrids localized the gene to Xq22-q28.