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LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1)

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RDL; RNA polymerase-associated protein LEO1; Replicative senescence down-regulated leo1-like protein

LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1)
CCC M433
HGNC 30401
MGI 2685031
PubMed 15632063
RGD 1549772
UniProt Q8WVC0
Wiki LEO1
LEO1, parafibromin (CDC73), CTR9, and PAF1 form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A and with a histone methyltransferase complex. The deduced 666-amino acid protein has an N-terminal AAA domain and a C-terminal domain that shares a high degree of similarity with yeast Leo1. LEO1 has several putative phosphorylation sites and several putative nuclear localization signals in the C terminus. The AAA domain was found in mouse, rat, and human LEO1 proteins but not in yeast, nematode, or fly Leo1 homologs. Northern blot analysis detected a 2.4-kb transcript highly expressed in skeletal muscle and heart, and more weakly expressed in placenta, liver, and other tissues. Fluorescence-tagged LEO1 localized predominantly in the nucleus of transfected human fibroblasts.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) CLIA Kit Customized Service Offer
n/a ELISA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) CLIA Kit Customized Service Offer
n/a ELISA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) CLIA Kit Customized Service Offer
n/a ELISA Kit for LEO1, Paf1/RNA Polymerase II Complex Component, Homolog (LEO1) ELISA Kit Customized Service Offer
  1. "Expression of the Leo1-like domain of replicative senescence down-regulated Leo1-like (RDL) protein promotes senescence of 2BS fibroblasts."FASEB J. 19:521-532(2005) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The parafibromin tumor suppressor protein is part of a human Paf1 complex."Mol. Cell. Biol. 25:612-620(2005) [PubMed] [Europe PMC] [Abstract]
  5. "Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo."Cell 125:327-341(2006) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  11. "A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity." Cell Stem Cell 4:403-415(2009) [PubMed] [Europe PMC] [Abstract]
  12. "DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation."Genes Dev. 23:2765-2777(2009) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  14. "The human PAF1 complex acts in chromatin transcription elongation both independently and cooperatively with SII/TFIIS."Cell 140:491-503(2010) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  16. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  19. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]