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Targeting Protein For Xklp2 (TPX2)

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DIL2; p100; GD: C20orf1; HCA519; HCTP4; REPP86; Differentially expressed in cancerous and non-cancerous lung cells 2; Restricted expression proliferation-associated 100

Targeting Protein For Xklp2 (TPX2)
CCC M388
HGNC 1249
MGI 1919369
PubMed 22303466
RGD 1311894
UniProt Q9ULW0
Wiki TPX2
TPX2 coimmunoprecipitated specifically with STK6 (AURKA) from mitotic HeLa cell extracts. Conversely, STK6 could be detected in TPX2 immunoprecipitates. Binding studies demonstrated that the N terminus of TPX2 directly interacted with the C-terminal catalytic domain of STK6. Although kinase activity was not required for their interaction, TPX2 was readily phosphorylated by STK6. Upon small interfering RNA (siRNA)-mediated elimination of TPX2 from cells, the association of STK6 with the spindle microtubules was abolished, although its association with spindle poles was unaffected. Conversely, depletion of STK6 by siRNA had no detectable influence on the localization of TPX2. The full-length C20ORF1 cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Targeting Protein For Xklp2 (TPX2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Targeting Protein For Xklp2 (TPX2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Targeting Protein For Xklp2 (TPX2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Targeting Protein For Xklp2 (TPX2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Targeting Protein For Xklp2 (TPX2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Targeting Protein For Xklp2 (TPX2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Targeting Protein For Xklp2 (TPX2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Targeting Protein For Xklp2 (TPX2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Targeting Protein For Xklp2 (TPX2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Targeting Protein For Xklp2 (TPX2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Targeting Protein For Xklp2 (TPX2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Targeting Protein For Xklp2 (TPX2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Targeting Protein For Xklp2 (TPX2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Targeting Protein For Xklp2 (TPX2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Targeting Protein For Xklp2 (TPX2) ELISA Kit Customized Service Offer
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  2. "Assignment of human proliferation associated p100 gene (C20orf1) to human chromosome band 20q11.2 by in situ hybridization."Cytogenet. Cell Genet. 84:182-183(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
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  6. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  7. "TPX2, a novel Xenopus MAP involved in spindle pole organization."J. Cell Biol. 149:1405-1418(2000) [PubMed] [Europe PMC] [Abstract]
  8. "p100: a novel proliferation-associated nuclear protein specifically restricted to cell cycle phases S, G2, and M."Blood 90:226-233(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  10. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A."J. Cell Biol. 182:289-300(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  14. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase."J. Cell Sci. 122:644-655(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  20. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Structural basis of Aurora-A activation by TPX2 at the mitotic spindle."Mol. Cell 12:851-862(2003) [PubMed] [Europe PMC] [Abstract]
  22. "Modulation of kinase-inhibitor interactions by auxiliary protein binding: crystallography studies on Aurora A interactions with VX-680 and with TPX2."Protein Sci. 17:1791-1797(2008) [PubMed] [Europe PMC] [Abstract]
  23. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]