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THO Complex Subunit 1 (THOC1)

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HPR1; P84; P84N5; Nuclear matrix protein p84

THO Complex Subunit 1 (THOC1)
CCC M360
HGNC 19070
MGI 1919668
PubMed 15870275
UniProt Q96FV9
Wiki THOC1
HPR1 is part of the TREX (transcription/export) complex, which includes TEX1, THO2, ALY, and UAP56.The predicted protein contains 657 amino acids and is conserved from yeast to humans.
Immunoblot analysis showed expression of an 84-kD protein. Confocal microscopy demonstrated a nuclear matrix localization, specifically as speckles in the RNA-processing centers. Northern blot analysis revealed expression of a 2.1-kb transcript. Immunoprecipitation analysis of mouse tissues and human cell lines indicated ubiquitous expression of p84 and RB1, suggesting a role in regulating a basic function.TREX complex is specifically recruited to the transcribing gene and travels with the polymerase during transcriptional elongation.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPM360Hu01 Recombinant THO Complex Subunit 1 (THOC1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to THO Complex Subunit 1 (THOC1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to THO Complex Subunit 1 (THOC1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for THO Complex Subunit 1 (THOC1) CLIA Kit Customized Service Offer
n/a ELISA Kit for THO Complex Subunit 1 (THOC1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant THO Complex Subunit 1 (THOC1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to THO Complex Subunit 1 (THOC1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to THO Complex Subunit 1 (THOC1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for THO Complex Subunit 1 (THOC1) CLIA Kit Customized Service Offer
n/a ELISA Kit for THO Complex Subunit 1 (THOC1) ELISA Kit Customized Service Offer
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  2. "The death domain protein p84N5, but not the short isoform p84N5s, is cell cycle-regulated and shuttles between the nucleus and the cytoplasm."FEBS Lett. 574:13-19(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 18." Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Apoptosis induced by the nuclear death domain protein p84N5 is inhibited by association with Rb protein."Mol. Biol. Cell 10:3251-3261(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Apoptosis induced by the nuclear death domain protein p84N5 is associated with caspase-6 and NF-kappa B activation."J. Biol. Chem. 275:25336-25341(2000) [PubMed] [Europe PMC] [Abstract]
  8. "The nuclear death domain protein p84N5 activates a G2/M cell cycle checkpoint prior to the onset of apoptosis."J. Biol. Chem. 276:1127-1132(2001) [PubMed] [Europe PMC] [Abstract]
  9. "TREX is a conserved complex coupling transcription with messenger RNA export."Nature 417:304-308(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Nuclear localization is required for induction of apoptotic cell death by the Rb-associated p84N5 death domain protein."Oncogene 21:4691-4695(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Linking transcriptional elongation and messenger RNA export to metastatic breast cancers."Cancer Res. 65:3011-3016(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Recruitment of the human TREX complex to mRNA during splicing."Genes Dev. 19:1512-1517(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Human hHpr1/p84/Thoc1 regulates transcriptional elongation and physically links RNA polymerase II and RNA processing factors."Mol. Cell. Biol. 25:4023-4033(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Human mRNA export machinery recruited to the 5' end of mRNA."Cell 127:1389-1400(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Recruitment of the complete hTREX complex is required for Kaposi's sarcoma-associated herpesvirus intronless mRNA nuclear export and virus replication."PLoS Pathog. 4:E1000194-E1000194(2008) [PubMed] [Europe PMC] [Abstract]
  17. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  18. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  20. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Genome instability and transcription elongation impairment in human cells depleted of THO/TREX."PLoS Genet. 7:E1002386-E1002386(2011) [PubMed] [Europe PMC] [Abstract]
  22. "Aly and THO are required for assembly of the human TREX complex and association of TREX components with the spliced mRNA."Nucleic Acids Res. 41:1294-1306(2013) [PubMed] [Europe PMC] [Abstract]
  23. "The Thoc1 encoded ribonucleoprotein is a substrate for the NEDD4-1 E3 ubiquitin protein ligase."PLoS ONE 8:E57995-E57995(2013) [PubMed] [Europe PMC] [Abstract]
  24. "Solution structure of the death domain of nuclear matrix protein p84."Submitted (JUL-2005) to the PDB data bank