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Trimethylguanosine Synthase (TGS1)

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NCOA6IP; PIMT; PIPMT; Nuclear Receptor Coactivator 6 Interacting Protein; Cap-specific guanine-N2 methyltransferase; Hepatocellular carcinoma-associated antigen 137

Trimethylguanosine Synthase (TGS1)
CCC M357
HGNC 17843
MGI 2151797
PubMed 22982455
UniProt Q96RS0
Wiki TGS1
The PIMT cDNA encodes an 852-amino acid protein containing a 9-amino acid methyltransferase motif I (VVDAFCGVG) and an invariant segment (GXXGXXI) found in K-homology motifs of many RNA-binding proteins. Northern blot analysis demonstrated ubiquitous expression of a 3.2-kb PIMT transcript, with highest expression in heart, skeletal muscle, kidney, liver, and placenta. PIMT and PRIP proteins are colocalized in the nucleus. PIMT binds 5-adenosyl-L-methionine, the methyl donor for the methyltransfer reaction, and it also binds RNA, suggesting that it is an RNA methyltransferase. Overexpression of PIMT enhances the transcriptional activity of PPARG and RXR, and this enhancement is further stimulated by overexpression of PRIP, PIMT is a component of nuclear receptor signal transduction that acts through PRIP.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Trimethylguanosine Synthase (TGS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Trimethylguanosine Synthase (TGS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Trimethylguanosine Synthase (TGS1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Trimethylguanosine Synthase (TGS1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Trimethylguanosine Synthase (TGS1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Trimethylguanosine Synthase (TGS1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Trimethylguanosine Synthase (TGS1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Trimethylguanosine Synthase (TGS1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Trimethylguanosine Synthase (TGS1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Trimethylguanosine Synthase (TGS1) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of PIMT, a protein with a methyltransferase domain, which interacts with and enhances nuclear receptor coactivator PRIP function."Proc. Natl. Acad. Sci. U.S.A. 98:10380-10385(2001) [PubMed] [Europe PMC] [Abstract]
  2. "Identification of tumor-associated antigens in chronic lymphocytic leukemia by SEREX."Blood 100:2123-2131(2002) [PubMed] [Europe PMC] [Abstract]
  3. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments."EMBO J. 21:2736-2745(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Interaction of PIMT with transcriptional coactivators CBP, p300, and PBP differential role in transcriptional regulation."J. Biol. Chem. 277:20011-20019(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localize to the cytoplasm and nucleus."Biochem. Biophys. Res. Commun. 309:44-51(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Interaction between the small-nuclear-RNA cap hypermethylase and the spinal muscular atrophy protein, survival of motor neuron."EMBO Rep. 4:616-622(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies."Mol. Biol. Cell 17:3221-3231(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Genetic and biochemical analysis of yeast and human cap trimethylguanosine synthase: functional overlap of 2,2,7-trimethylguanosine caps, small nuclear ribonucleoprotein components, pre-mRNA splicing factors, and RNA decay pathways."J. Biol. Chem. 283:31706-31718(2008) [PubMed] [Europe PMC] [Abstract]
  13. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  17. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1."Acta Crystallogr. D 65:332-338(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1."Nucleic Acids Res. 37:3865-3877(2009) [PubMed] [Europe PMC] [Abstract]