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TBC1 Domain Family, Member 4 (TBC1D4)

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AS160; Akt substrate of 160 kDa

TBC1 Domain Family, Member 4 (TBC1D4)
CCC M344
HGNC 19165
MGI 2429660
PubMed 22466139
UniProt O60343
Wiki TBC1D4
The 160 kD protein product was first discovered in a screen for novel substrates of the serine-threonine kinase Akt, which phosphorylates AS160 after insulin stimulation. Insulin stimulation of fat and muscle cells results in translocation of the glucose transporter GLUT4 to the plasma membrane, and this translocation process is dependent on phosphorylation of AS160. The role of AS160 in GLUT4 translocation is mediated by its GTPase activating domain and interactions with Rab proteins in vesicle formation, increasing GLUT4 translocation when its GTPase activity is inhibited by Akt phosphorylation. Specifically, this inhibition activates RAB2A, RAB8A, RAB10 and RAB14.AS160 also contains a calmodulin-binding domain, and this domain mediates phosphorylation-independent glucose uptake in muscle cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TBC1 Domain Family, Member 4 (TBC1D4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TBC1 Domain Family, Member 4 (TBC1D4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TBC1 Domain Family, Member 4 (TBC1D4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TBC1 Domain Family, Member 4 (TBC1D4) CLIA Kit Customized Service Offer
n/a ELISA Kit for TBC1 Domain Family, Member 4 (TBC1D4) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant TBC1 Domain Family, Member 4 (TBC1D4) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to TBC1 Domain Family, Member 4 (TBC1D4) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to TBC1 Domain Family, Member 4 (TBC1D4) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for TBC1 Domain Family, Member 4 (TBC1D4) CLIA Kit Customized Service Offer
n/a ELISA Kit for TBC1 Domain Family, Member 4 (TBC1D4) ELISA Kit Customized Service Offer
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  15. "Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects."Diabetes 54:1692-1697(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
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  18. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
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  20. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  22. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  23. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  24. "Rab10 and myosin-Va mediate insulin-stimulated GLUT4 storage vesicle translocation in adipocytes."J. Cell Biol. 198:545-560(2012) [PubMed] [Europe PMC] [Abstract]
  25. "A common Greenlandic TBC1D4 variant confers muscle insulin resistance and type 2 diabetes." Nature 512:190-193(2014) [PubMed] [Europe PMC] [Abstract]
  26. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  27. "Crystal structures of human TBC1D1 and TBC1D4 (AS160) RabGTPase-activating protein (RabGAP) domains reveal critical elements for GLUT4 translocation."J. Biol. Chem. 286:18130-18138(2011) [PubMed] [Europe PMC] [Abstract]