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Serine/Arginine Repetitive Matrix Protein 2 (SRRM2)

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SRL300; SRm300; 300 kDa nuclear matrix antigen; Serine/arginine-rich splicing factor-related nuclear matrix of 300 kDa; Tax-responsive enhancer element-binding protein 803

Serine/Arginine Repetitive Matrix Protein 2 (SRRM2)
CCC M292
HGNC 16639
MGI 1923206
PubMed 20161708
RGD 1310163
UniProt Q9UQ35
The deduced 2,752-amino acid protein has multiple R, S, and P residues, numerous phosphorylation sites, and a predicted molecular mass of 300 kD, suggesting that it may be the full-length protein. Immunoblot analysis detected GST fusion proteins of greater than 300 kD in human and rat cells. Northern blot analysis revealed expression of a 9.0- to 10.0-kb SRL300 transcript in all tissues and cell lines tested.Like SRM160, the deduced 2,296-amino acid SRM300 protein is rich in serine (S), arginine (R), and proline (P), has numerous SR dipeptides and 2 long polyserine domains, and lacks an RNA recognition domain. Reconstitution of SRM160/SRM300-depleted splicing reactions with recombinant SRM160 restored splicing activity, suggesting that SRM160 is the more important component of the complex.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Serine/Arginine Repetitive Matrix Protein 2 (SRRM2) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of a novel RNA-binding protein SRL300 with RS domains."Biochim. Biophys. Acta 1492:191-195(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The SRm160/300 splicing coactivator subunits."RNA 6:111-120(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 4:141-150(1997) [PubMed] [Europe PMC] [Abstract]
  4. "The sequence and analysis of duplication-rich human chromosome 16." Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. , Submitted (JUL-2007) to UniProtKB
  7. "A coactivator of pre-mRNA splicing."Genes Dev. 12:996-1009(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  11. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  13. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  14. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  15. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome."RNA 15:2161-2173(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  21. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  22. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  23. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  24. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  25. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]