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Specificity Protein 1 (Sp1)

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TSFP1; Sp1 Transcription Factor

Specificity Protein 1 (Sp1)
CCC M264
HGNC 11205
MGI 98372
PubMed 23250796
RGD 3738
UniProt P08047
Wiki SP1
Sp1 is a human transcription factor involved in gene expression in the early development of an organism. It belongs to the Sp/KLF family of transcription factors. The protein is 785 amino acids long, with a molecular weight of 81 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and binds the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.Sp1 has been used as a control protein to compare with when studying the increase or decrease of the aryl hydrocarbon receptor and/or the estrogen receptor, since it binds to both and generally remains at a relatively constant level.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPM264Hu01 Recombinant Specificity Protein 1 (Sp1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAM264Hu01 Polyclonal Antibody to Specificity Protein 1 (Sp1) WB; IHC; ICC; IP.
Assay Kits SEM264Hu ELISA Kit for Specificity Protein 1 (Sp1) Enzyme-linked immunosorbent assay for Antigen Detection.
HEM264Hu High Sensitive ELISA Kit for Specificity Protein 1 (Sp1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Specificity Protein 1 (Sp1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Specificity Protein 1 (Sp1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Specificity Protein 1 (Sp1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Specificity Protein 1 (Sp1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Specificity Protein 1 (Sp1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Specificity Protein 1 (Sp1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Specificity Protein 1 (Sp1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Specificity Protein 1 (Sp1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Specificity Protein 1 (Sp1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Specificity Protein 1 (Sp1) ELISA Kit Customized Service Offer
  1. "Identification of a novel Sp1 splice variant as a strong transcriptional activator."Biochem. Biophys. Res. Commun. 412:86-91(2011) [PubMed] [Europe PMC] [Abstract]
  2. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing."J. Biol. Chem. 275:38067-38072(2000) [PubMed] [Europe PMC] [Abstract]
  5. "Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain."Cell 51:1079-1090(1987) [PubMed] [Europe PMC] [Abstract]
  6. "O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation."Cell 55:125-133(1988) [PubMed] [Europe PMC] [Abstract]
  7. "Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif."Cell 55:887-898(1988) [PubMed] [Europe PMC] [Abstract]
  8. "Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat."J. Biol. Chem. 270:25564-25569(1995) [PubMed] [Europe PMC] [Abstract]
  9. "The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1."J. Biol. Chem. 271:4417-4430(1996) [PubMed] [Europe PMC] [Abstract]
  10. "O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions."Mol. Cell. Biol. 17:6472-6480(1997) [PubMed] [Europe PMC] [Abstract]
  11. "The SV40 capsid protein VP3 cooperates with the cellular transcription factor Sp1 in DNA-binding and in regulating viral promoter activity."J. Mol. Biol. 275:187-195(1998) [PubMed] [Europe PMC] [Abstract]
  12. "Functional interactions between Sp1 or Sp3 and the helicase-like transcription factor mediate basal expression from the human plasminogen activator inhibitor-1 gene."J. Biol. Chem. 274:19573-19580(1999) [PubMed] [Europe PMC] [Abstract]
  13. "A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening."Mol. Cell. Biochem. 210:131-142(2000) [PubMed] [Europe PMC] [Abstract]
  14. "O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability."Proc. Natl. Acad. Sci. U.S.A. 98:6611-6616(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Identification of two Sp1 phosphorylation sites for p42/p44 mitogen-activated protein kinases: their implication in vascular endothelial growth factor gene transcription."J. Biol. Chem. 277:20631-20639(2002) [PubMed] [Europe PMC] [Abstract]
  16. "Cellular transcription factor Sp1 recruits simian virus 40 capsid proteins to the viral packaging signal, ses."J. Virol. 76:5915-5924(2002) [PubMed] [Europe PMC] [Abstract]
  17. "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter."J. Biol. Chem. 278:36496-36504(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Interaction between the varicella zoster virus IE62 major transactivator and cellular transcription factor Sp1."J. Biol. Chem. 278:38068-38075(2003) [PubMed] [Europe PMC] [Abstract]
  19. "Fibroblast growth factor-2 represses platelet-derived growth factor receptor-alpha (PDGFR-alpha) transcription via ERK1/2-dependent Sp1 phosphorylation and an atypical cis-acting element in the proximal PDGFR-alpha promoter."J. Biol. Chem. 279:2377-2382(2004) [PubMed] [Europe PMC] [Abstract]
  20. "Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins."J. Biol. Chem. 280:13928-13935(2005) [PubMed] [Europe PMC] [Abstract]
  21. "Insulin dynamically regulates calmodulin gene expression by sequential O-glycosylation and phosphorylation of SP1 and its subcellular compartmentalization in liver cells."J. Biol. Chem. 281:3642-3650(2006) [PubMed] [Europe PMC] [Abstract]
  22. "HER-2/neu represses the metastasis suppressor RECK via ERK and Sp transcription factors to promote cell invasion."J. Biol. Chem. 281:4718-4725(2006) [PubMed] [Europe PMC] [Abstract]
  23. "Sumoylation inhibits cleavage of Sp1 N-terminal negative regulatory domain and inhibits Sp1-dependent transcription."J. Biol. Chem. 281:5567-5574(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Increased chromatin association of Sp1 in interphase cells by PP2A-mediated dephosphorylations."J. Mol. Biol. 364:897-908(2006) [PubMed] [Europe PMC] [Abstract]
  25. "Sp1 deacetylation induced by phorbol ester recruits p300 to activate 12(S)-lipoxygenase gene transcription."Mol. Cell. Biol. 26:1770-1785(2006) [PubMed] [Europe PMC] [Abstract]
  26. "Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression of transcription of the luteinizing hormone receptor gene."Mol. Cell. Biol. 26:6748-6761(2006) [PubMed] [Europe PMC] [Abstract]
  27. ErratumMol. Cell. Biol. 26:8214-8214(2006)
  28. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  29. "Phosphorylation of Sp1 in response to DNA damage by ataxia telangiectasia-mutated kinase."Mol. Cancer Res. 5:1319-1330(2007) [PubMed] [Europe PMC] [Abstract]
  30. "Activation of PPARgamma negatively regulates O-GlcNAcylation of Sp1."Biochem. Biophys. Res. Commun. 372:713-718(2008) [PubMed] [Europe PMC] [Abstract]
  31. "Phosphorylation mediates Sp1 coupled activities of proteolytic processing, desumoylation and degradation."Cell Cycle 7:623-630(2008) [PubMed] [Europe PMC] [Abstract]
  32. "Identification of phosphorylation sites on transcription factor Sp1 in response to DNA damage and its accumulation at damaged sites."Cell. Signal. 20:1795-1803(2008) [PubMed] [Europe PMC] [Abstract]
  33. "Angiotensin II-inducible platelet-derived growth factor-D transcription requires specific Ser/Thr residues in the second zinc finger region of Sp1."Circ. Res. 102:38-51(2008) [PubMed] [Europe PMC] [Abstract]
  34. "Phosphorylation by c-Jun NH2-terminal kinase 1 regulates the stability of transcription factor Sp1 during mitosis."Mol. Biol. Cell 19:1139-1151(2008) [PubMed] [Europe PMC] [Abstract]
  35. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  36. "O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors."Biochem. Biophys. Res. Commun. 380:569-574(2009) [PubMed] [Europe PMC] [Abstract]
  37. "O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y."Biochem. Biophys. Res. Commun. 382:593-597(2009) [PubMed] [Europe PMC] [Abstract]
  38. "MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]
  39. "O-linked N-acetylglucosaminylation of Sp1 inhibits the human immunodeficiency virus type 1 promoter."J. Virol. 83:3704-3718(2009) [PubMed] [Europe PMC] [Abstract]
  40. "Human BAHD1 promotes heterochromatic gene silencing."Proc. Natl. Acad. Sci. U.S.A. 106:13826-13831(2009) [PubMed] [Europe PMC] [Abstract]
  41. "Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b."FEBS J. 277:1202-1218(2010) [PubMed] [Europe PMC] [Abstract]
  42. "Transcriptional regulation of human FE65, a ligand of Alzheimer's disease amyloid precursor protein, by Sp1."J. Cell. Biochem. 109:782-793(2010) [PubMed] [Europe PMC] [Abstract]
  43. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  44. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  45. "Transforming growth factor-beta-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs."Cell. Mol. Life Sci. 68:1953-1968(2011) [PubMed] [Europe PMC] [Abstract]
  46. "Cooperative transcriptional activation by Klf4, Meis2, and Pbx1."Mol. Cell. Biol. 31:3723-3733(2011) [PubMed] [Europe PMC] [Abstract]
  47. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  48. "Structures of zinc finger domains from transcription factor Sp1. Insights into sequence-specific protein-DNA recognition."J. Biol. Chem. 272:7801-7809(1997) [PubMed] [Europe PMC] [Abstract]